与杨树木材密度、纤维性状相关的SSR分子标记

SSR Molecular Markers Related to Wood Density and Fibre Traits in Poplar

通过对美洲黑杨 (Populusdeltoides)×青杨 (P .cathayana)的 68个F2 、双亲及其 4个F1无性系田间随机区组设计 ,5次重复 ,分析了与木材材性有关的木材密度、纤维长、纤维宽以及微纤丝角等性状。结果表明 :纤维长有明显的杂种优势 ,控制该性状表现的基因之间具有正效应的互作 ;控制木材基本密度的基因之间具有负效应的互作。结合SSR分子标记结果 ,采用单因素方差分析法进行标记与性状的相关分析 ,共找到与木材密度、纤维长、纤维宽以及微纤丝角相关联的标记分别为 5、7、4、2个 ;青杨在这 4个材性性状方面也具有对性状起贡献的标记 ,如与木材密度相关的PMGC2 873 1(贡献率 4 88% )标记 ,与纤维长相关的PMGC456 3 (贡献率 2 2 96% )、PMGC2 70 2 2 (贡献率9 17% )标记 ,与纤维宽相关的PMGC2 40 8 1(贡献率 7 18% )标记 ,与微纤丝角相关PMGC2 52 5 1(贡献率 16 59% )标记 ,在育种中如果能正确加以利用 。

In this study,field experiment was carried out following the randomized complete block design with five replications in 1999.F_2 population was derived from a cross between the female ZH3 and the male ZH1 (ZH3,ZH1 were selected from F_1 individuals derived from the cross of Populus deltoides with P.cathayana,produced by HUANG Dong-Sen in 1973).Sixty-eight F_2 populations,four F_1 individuals (included ZH3,ZH1) and both parents (Populus deltoides and P.cathayana) were measured for wood densities,fibre lengths,fibre widths and fibre angles.The results indicated that the trait of fibre length has obviously heterosis,there may have positive effects among the genes controlling the trait of fibre length,but may have negative effects among the genes controlling the trait of wood density.Using the method of single factor variance,the SSR markers correlated with wood density,fibre length,fibre width and fibre angle were identified to be 5,7,4 and 2,respectively.There were some useful markers among the above traits in P.cathayana,such as PMGC2873-1 for wood density (its contribution was 4.88%),PMGC456-3,PMGC2702-2 for fibre length (their contribution were 22.96% and 9.17%,respectively),PMGC2408-1 for fibre width (its contribution was 7.18%),and PMGC2525-1 for fibre angle (its contribution was 16.59%),these markers might be useful for wood property improvement if using them correctly in breeding program.