摘要
低氧诱导因子 - 1(HIF 1)是一种由低氧诱导因子 - 1α (HIF 1α)和芳香烃核受体转录因子(ARNT)组成的二聚体转录因子 ,在生物体氧平衡调节中起关键作用。高原鼠兔 (Ochotonacurzoniae)是生活在青藏高原海拔 30 0 0m以上区域 ,有着极强的低氧、低温耐受能力的特有自然种群。它通过高的基础代谢率和高的氧利用率来适应高原低温、缺氧环境。将高原鼠兔带到北京 ,常氧室温条件下适应 7d。RT PCR扩增高原鼠兔HIF 1αcDNA片断 ,3 2 P标记作为特异性探针 ,Northern杂交检测了常氧条件下高原鼠兔HIF 1α的组织特异性表达。结果显示 ,HIF 1αmRNA在高原鼠兔多种组织中均有表达 ,并且表现出明显的组织差异性。其中脑的表达量最大 ,肾其次 ,心、脾和肝的表达相对较少。
Hypoxia inducible factor 1 (HIF 1),which is a transcription factor composed of HIF 1α and arylhydocarbon nuclear receptor translocator (ARNT),plays an important role in the signal transduction of cell response to hypoxia.Plateau pikas ( Ochotona curzoniae ),which are a kind of high anoxia tolerance and cold adaptation species lived only above 3 000 m on Qinhai Tibet Plateau,have unusual high RMR and NST,acting mainly via increasing NST to adapt to extreme cold of the Qinghai Tibet Plateau;and high ratio of oxygen utilizing to cope with plateau hypoxia environment.In this study,the plateau pikas were taken to Beijing and adapted under normal oxygen and room temperature for 7 days.One fragment of the plateau pika HIF 1α cDNA was obtained by RT PCR technique and labeled with 32 P as specific probe.Northern blot analysis showed that HIF 1α mRNA was expressed in many kinds of tissues and displayed a tissue specific expression:the highest in brain,next in kidney,and much less in heart,liver,and spleen.
基金
中国科学院知识创新工程重大项目实验站观测专题 (KZCX1-SW -0 1-0 1A5 )
中国科学院创新重大项目课题(KZCX1-0 9-0 1)
中科院海北高寒草甸生态系统开放试验站基金项目
