摘要
用 18 0条引物对黔东南小香羊、贵州白山羊、贵州黑山羊和黔北麻羊 4个贵州地方山羊品种 (种群 ) ,以及南江黄羊和波尔山羊进行RAPD分析 ,其中 2 7条引物扩增出多态性图谱。这 2 7条引物共扩增出 2 81条带 ,多态带为 115条 ,平均多态频率为 4 0 92 % (范围 2 0 %～ 80 % ) ;每条引物平均扩增条带为 10 4 1条 (范围 4～ 16条 ) ;扩增带分子量在 2 10～ 2 80 0bp。贵州白山羊与贵州黑山羊之间的遗传距离指数 ( 0 0 6 0 5 )最小 ,而波尔山羊与其他品种之间的遗传距离指数 ( 0 10 5 9～ 0 14 88)最大。NJ法聚类结果显示 ,贵州白山羊与贵州黑山羊间的亲缘关系最近 ,其次为黔北麻羊 ,而黔东南小香羊与其他 3个贵州地方品种的亲缘关系较南江黄羊还远。分析表明 ,黔东南小香羊在遗传上为一独立的品种 ;而贵州地方山羊品种间具有较近的亲缘关系 ,遗传变异较小 。
We used 180 primers in RAPD analysis of Guizhou native goats (the small xiang goat,Guizhou white goat,Guizhou black goat and Qianbei pockmarked goat),Nanjiang brown goats and Boer goats.Of the primers,27 amplified polymorphic patterns,including 281 bands.Of the bands,115 were polymorphic and their frequency was 40 92% on average (range 20%-80%).Each of the 27 primers had 10 41 bands on average (range 4-16),and the fragment length of products was 210-2?800?bp.Genetic distance index appeared the lowest between the Guizhou black goat and Guizhou white goat (0 0605),and the highest between the Boer goat and the other goats (0 1059-0 1488).NJ tree indicated that the Guizhou white goat was closely related to the Guizhou black goat,and the next was the Qianbei pockmarked goat;the small xiang goat was farther from the other Guizhou native goats than Nanjiang brown goat from.The results analysis suggest that the small xiang goat is a independent breed on genetics,and Guizhou native goat breeds have the close genetic relationship,low genetic variability and high genetic stability.
基金
贵州大学校基金(2 0 0 0 )
贵州省科学技术基金 [黔基合计字(2 0 0 2 ) 3 0 5号 ]
国家自然科学基金资助项目(3 0 0 70 12 0 )
