黑鲷DMRT1基因cDNA的克隆、组织表达谱及在性别逆转前后性腺中的表达

cDNA Cloning,Tissue Distribution and mRNA Transcription of DMRT1 Gene in the Protandrous Black Porgy Acanthopagrus schlegeli

利用RT PCR和 3′ RACE的方法从黑鲷精巢中克隆了DMRT1基因cDNA的部分序列。组织特异性表达分析表明DMRT1基因只在精巢中表达。半定量RT PCR检测显示DMRT1基因在性别逆转前、性别逆转期和性别逆转后的精巢中的表达量无显著差异。在鱼类成体的精巢中 ,DMRT1的表达量可能不会因精巢结构或生理状态的改变而发生改变 。

Using RT PCR and 3′ RACE, DMRT1 gene was partially cloned from the black porgy, Acanthopagrus schlegeli .RT PCR showed that DMRT1 mRNA was only expressed in the testis.When the transcript levels of DMRT1 were measured in the testis of black porgy before sex change,during sex change,or after sex change,no significant changes in the gene expression were observed.The results show that the transcript levels of DMRT1 gene in the testis of black porgy don't vary according to the changes of physiological status and structure of the testis.