摘要
本文报道对中国新疆东部一起戊型肝炎(HE)流行中2例HE患者粪便中分离到的HEV中国XT-179株系基因组进行cDNA分子克隆和其免疫原性表位的血清学研究结果。cDNA序列分析表明中国XT-179株与缅甸ET1.1株之间有94.3％的核苷酸和99.6％的氨基酸具有同源性。但也有5.7％的核苷酸区别于其他HEV地理株和散发株,提示在中国流行型HEV可能存在独特的基因型,可将此毒株序列作为鉴别HEV不同毒株的病毒学依据之一。本研究还按上述HEV地理株之间共有的免疫原性表位氨基酸序列合成了多肽抗原,用于检测来自中国XT-179株系流行区的29例HEV感染者和8例正常人血清抗-HEV,与应用HEV中国XT-179株系颗粒抗原相比,两组血清抗-HEV阳性和阴性的符合率分别为92.6％和100％。证实本项研究所克隆的HEV基因组可用于制备HEV重组基因疫苗和免疫诊断制剂。
The fragment genome of hepatitis E viruses were cloned as cDNAs, two from an epidemic outbseak of hepatitis E in the East Xinjiang of China(named HEV China XT-179 strain). cDNA sequence comparison was performed in the. 3'-terminal region, which showed 94.3% homology at nucleotide level and 99.6% homology at the amino acid level, approximally 820 base long between HEV China XT-179 and Myanmar ET1.1 strains. The peptide antigen was synthesed as their same amino acid sequence of immunoreactive epitopes, and also reacted with 29 sera of patients with hepatitis and 8 sera of normal people from the East Xinjiang of China when compared with that of HEV particle antigen of China XT-179 strain. The results showed that positive reaction with the sera of patients and nagtive reaction with normal sera coincident rate was 92.6% and 100%. These results obviously demonstrates in that recombinant DNA can be used for the development of detection system and vaccine of prevention of HEV infection and identification of virological link of different geographical locations.
