两种黄海潮间带海绵的元素与氨基酸成分分析

Elemental and total amino acid composition of two intertidal sponges in Yellow Sea

应用能量色散X射线荧光光谱 (EDXRF)分析了黄海潮间带繁茂膜海绵 (Hymeniacidonperleve)和肾指海绵 (Reniochalinasp.)的无机元素,其中硅是两种海绵中最多的无机元素。繁茂膜海绵较肾指海绵的锌含量高 ,参照文献数据推测这可能是该属海绵的特性。测定了2种海绵的含水量 ,有机碳、氮、氢元素和氨基酸在海绵干质量中的比例。分析了繁茂膜海绵在2002年8月到11月中主要无机元素的变化 ,发现繁茂膜海绵中的硅含量在降低。繁茂膜海绵的氨基酸总量远高于肾指海绵 ,2种海绵中含量较高的氨基酸是甘氨酸、谷氨酸、天冬氨酸、亮氨酸、缬氨酸、赖氨酸等。元素与氨基酸分析将对海绵细胞培养基开发有帮助。

The inorganic elemental compositions of two marine sponges(Hymeniacidon perleve and Reniochalina sp.)in the intertidal zone in YellowSea(China)were analysed by energy-dispersive X-ray fluorescence spectrometry(EDXRF).Silicon was the highest inorganic element in this sponge.Zinc in Hymeniacidon perleve was much higher than that of Reniochalina sp.Compared with the data of Araujo(1999,Chemical Speciation and Bioavailability,11,25-36),it may be the nature of this genus,Hymeniacidon sp.The radio of water in collected sponge;organic elements(carbon,nitrogen and hydrogen)and total amino acid compositions of these sponges in dry weight were also measured.Several inˉorganic element of Hymeniacidon perleve are determined by EDXRF from August to November2002.At the same time,silicon in sponge was decreased.The total amino acids of Hymeniacidon sp.were also higher than that of Reniochalina sp.Both kinds of sponge,were vich in glycine,glutamic acid,aspartic acid,leucine,valine and lysine.These information would helpful in medium design of marine sponge cell culture in vitro.