大弹涂鱼不同组织器官的同工酶研究

Tissue-specificity of the isozymes in various parts of Boleophthalmus pectinirostris

采用垂直聚丙烯酰胺平板电泳技术 ,对大弹涂鱼(Boleophthalmuspectinirostris)的眼睛、心脏、肝脏、肌肉、脾脏和鳃等6种组织器官中的13种同工酶进行研究 ,并分析了其酶谱表型。结果表明,四唑氧化酶 (TO)和酸性磷酸酶 (ACP)没有检测到酶带 ;三梨醇脱氢酶 (SDH)和葡萄糖-6-磷酸脱氢酶 (G -6 -PDH)仅在肝脏中表达 ;超氧化物歧化酶 (SOD)在所有组织中均存在 ,部分组织中存在差异 ;乳酸脱氢酶 (LDH)、苹果酸脱氢酶 (MDH)、苹果酸酶 (MEP)、酯酶(EST)、醇脱氢酶 (ADH)、D -葡萄糖脱氢酶 (GcDH)、甲酸脱氢酶 (FDH)、谷氨酸脱氢酶 (GDH)则具有明显的组织特异性。

Thirteen isozymes in six tissues or organs:eyes,gill,spleen,liver,muscle,and heart of Boleophthalmus pectinirostris were studied by using the vertical polyacrylamide gel electrophoresis,the phenotypes and the expressions of the isozymes were analyzed.The results showed that ACP and TO were not detected in all organs,SDH and G-6-PDH isozymes expressed only in the liver.The SOD isozyme existed in all these tissues,the activity of the SOD in liver was higher than other tissues.The LDH、MDH、MEP、ESTADH、GcDH、FDH and GDH isozymes were dearly tissue-specific.Namely,the LDH-A 4 isozyme was active in muscle,LDH-B 4 active in heart,but LDH-C 4 was observed only in eye,concerned with apparent tissues-specificity.