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小剂量阿糖胞苷诱导HL-60细胞分化的作用

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摘要 目的观察小剂量阿糖胞苷对HL 6 0细胞生长与分化的影响及其可能的作用机制。方法阿糖胞苷 (10 8M)作用HL 6 0细胞 2 6天 ,观察细胞生长 ,用流式细胞仪分析细胞周期 ,用免疫组化法检测P5 3、P2 1蛋白表达的变化 ,原位杂交法检测P5 3mRNA表达水平的改变。结果小剂量阿糖胞苷可诱导HL 6 0细胞向单核 /巨噬细胞分化 ,抑制HL 6 0细胞的增殖 ,使细胞停滞于G1期 ,P5 3,P2 1蛋白和P5 3mRNA表达明显增强。结论小剂量阿糖胞苷对HL 6 0细胞具增殖抑制和诱导分化作用 ,该作用与G1期阻滞及P5 3,P2 1表达增高有关。
作者 杜冰 唐恩洁 朱道银
机构地区 川北医学院分子生物学研究所 重庆医科大学微生物学教研室
出处 《川北医学院学报》 CAS 2004年第1期10-12,共3页 Journal of North Sichuan Medical College
关键词 小剂量 阿糖胞苷 诱导 HL—6O细胞 作用机制
分类号 R979.1 [医药卫生—药品]
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参考文献9

  • 1卓娅.细胞周期调控基因——p21^(CIP1/WAF1)[J].国外医学（分子生物学分册）,1998,20(1):6-9. 被引量：10
  • 2杜冰,朱道银,唐恩洁.重组人白血病抑制因子对HL-60细胞的作用及机制研究[J].重庆医科大学学报,2003,28(3):270-272. 被引量：5
  • 3李本尚.阿糖胞苷治疗急性白血病机理的研究进展[J].同济大学学报（医学版）,2002,23(1):68-71. 被引量：6
  • 4Sung KW,Choi HS,Yoo ES,et al. Clinical trial of low dose cytosine arabinoside in the treatment of acute promyelocytic leukemia[J].JKorean Med Sci, 1997;12(4):298-304.
  • 5Ossenkoppele CJ, Wijermans PW, de Waal FC, et al. Low dose cytosine-arabinoside has only minimal differentiation inducing capacity in HL60 cells[J]. Eur J Cancer Clin Oncol, 1989;25(11):1561-1569.
  • 6Faille A, Poirier O, Tunnel P, et al. Changes in thepatterns of protein phosphorylation associated with granulocytic and monocytic-induced differentiation of HL-60 ceils[J]. Anticancer Res,1986; 6(5) : 1053-1063.
  • 7Kizaki M, Dawson MI, Heyman R et al. Effect of novel retinoid receptor-selective ligands on myeloids leukemic differentiation and proliferation in vitro[J]. Blood, 1998 ; 87:1977-1983.
  • 8Li Y,Jenkins CW,Nichols MA,et al. Ceil cycle expression and P53 regulation Of the eye Un-dependent kinase inhibitor P21[J]. Oncogene, 1994;9(8):2261-2268.
  • 9Michieli P,Chedid M, Lin D, et al. Induction of WAF1/Cipl by a p53 independent pathway [J]. Cancer Res, 1994,54(13) :3391-3395.

二级参考文献13

  • 1[1]Bullock G,Ray S,Reed JC,et al.Intracellular me tabolism of Ara-C and resulting DNA fragmentation and apoptosis of human AML HL .60 cells possessing disparate levels of Bcl-2 protein[J].Leukemia,1996,10(4 ): 1731-1740.
  • 2[2]Freund,Rossig,Lanvers,et al.All-trans-retinoic acid increases cytosine arabinoside cytotoxicity in HL.60 human leukemia cells in sp ite of deceased cellular Ara-CTP accumulation[J].Annual oncology,1999,10(3):3 35-338.
  • 3[3]Koo HM,Mcwilliams MJ,Alvord WG,et al.Ras oncogene-ind uced sensitization to 1-beta-D-arabinofuranosylcytosine[J].Cancer Research, 1999,59(24):6057-6062.
  • 4[4]Meinhardt,Roth,Totok,et al.Signaling defect in the act ivation of caspase-3 and PKC delta in human cells is associated with resistance to apoptosis[J].Experimential cell research,1999,247(2):535-542.
  • 5[5]Viana,Kramer,Saunders,et al.Inhibition of protein kin ase C activator-mediated induction of P21C1P1 and P27K1P1 by deoxycytidine anal ogs in human leukemia cells:relationship to apoptosis and differentiation[J].B iochemical Pharmacology,1999,58 (1):121-131.
  • 6[6]Susan P,Whitmant,Trancesca.Protein kinase C activation by 1-D-arabinofuranosylcytosine is antagonistic to stimulation of apoptosis and Bcl-2 down-regulation[J].The journal of biological chemistry,1997,272(38): 2 3481-23484.
  • 7[7]Barbara H,Liebermann DA.The proto-oncogene c-Myc and apo ptosis[J].Oncogene,1998,17(25):3351-3357.
  • 8[8]Chelliah J,Freemerman AJ,Susanna WP,et al.Protentiat ion of Ara-C induced apoptosis by the protein kinase C activator bryostatin 1 i n human leukemia cells (HL.60) involves a process dependent upon c- Myc[J].B iochemical Pharmacology,1997,54:563-573.
  • 9[9]Kim CN,Wang XD.Overexertion of bcl-xl inhibits Ara-C-in duced mitochondrial loss of cytochrome c and other perturbations that activate t he molecular cascade of apoptosis[J].Cancer Research,1997,57:3115-3120.
  • 10[10]Marina K,Tari AM,Zeev E,et al. Loposomal Bcl-2 antisense oligonucle otides enhance proliferation,sensitize acute myeloid leukemia to cytosine-arabi noside,and induce apoptosis independent of other antiapoptotic proteins[J].Blo od,2000,95(12):3929-3938.

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川北医学院学报
2004年 第1期

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