转染丙型肝炎病毒非结构蛋白NS4B对P53表达的影响

Effects of non-structural protein NS4B of transfected hepatitis C virus on the expression of p53

目的了解丙型肝炎病毒非结构蛋白NS4B对肝细胞内p5 3表达的影响 ,探讨其在肝癌发生中的作用 ,及其发生机制。方法使用脂质体介导法 ,转染丙型肝炎病毒非结构蛋白重组质粒PCXN2 NS4B及突变型p5 3基因重组质粒P5 3 CX2 2AN3进入Chang肝细胞内 ,并用G4 18筛选获得稳定表达细胞 ,分别采用细胞化学法检测p5 3表达率。设置空白对照组、空白载体组、转染NS4B组 ,转染p5 3组、共转染NS4B及p5 3组。结果空白对照组无p5 3表达 ,空白载体组及转染NS4B组呈弱阳性表达 ,转染p5 3组及共转染组呈阳性表达 ,与空白对照组、空白载体组及转染NS4B组比较有显著差异 ,P <0 .0 1。结论NS4B可能抑制突变型p5 3表达 ,也可能阻止其进入细胞核。

Objective Investigate the effects of non--structural protein NS4B of hepatitis C ivirus on the expression of p53 in the hepatocyte. Methods The recombinant plasmids of hepatits C virus non--structural protein (PCXN2--NS4B) and mutant-type p52 were detected using immunocytoehemistry. Results Compared with controls, the expression of p53 was slightly positive in the vector group and NS4B-transiected group, and significantly positive in the p53-transfected group and both NS4B and p53 -transfected group. Conclusion NS4B might inhibit the expression of the mutant--type p53, or prevent it from entering the nucleus.