针刺对肥胖大鼠下丘脑神经肽含量的影响

Effect of Acupuncture on the Contents of Hypothalamic Neuropeptides in Obesity Rats

目的 :探讨针刺对肥胖机体下丘脑神经肽含量的影响。方法 :观察针刺治疗前后肥胖大鼠体重、Lee’s指数和体脂量以及下丘脑β 内啡肽 ( β EP)、α 促黑激素 (α MSH)、铃蟾肽 (BOM )、神经肽Y(NPY)、血管紧张素Ⅱ (ATⅡ )、心房肽 (ANP)、降钙素基因相关肽 (CGRP)的含量的变化。结果 :肥胖大鼠体重、Lee’s指数、体脂以及下丘脑β EP、NPY和AngⅡ的含量均显著高于正常大鼠水平 ,而下丘脑α MSH、BOM、ANP和CGRP的含量均显著低于正常大鼠水平。相关分析显示 ,下丘脑 β EP、NPY和AngⅡ的含量与肥胖指标呈正相关 ;而下丘脑α MSH、BOM、ANP和CGRP的含量与肥胖指标呈负相关。针刺治疗取得良好减肥疗效的同时 ,肥胖大鼠下丘脑 β EP、NPY和AngⅡ的含量明显回降 ,而下丘脑α MSH、BOM、ANP和CGRP的含量明显回升。结论 :生物机体下丘脑β EP、NPY和AngⅡ水平异常增高和下丘脑α MSH、BOM、ANP和CGRP的水平异常低下可能是产生肥胖的重要因素。针刺对肥胖机体下丘脑神经肽的良性调整作用可能是针刺减肥的重要作用机制。

Objective:To explore the effect of acupuncture on the contents of hypothalamic neuropeptides in obesity rats. Methods: 15 SD rats were randomly and evenly divided into control, model and acupuncture groups. Obesity model was established before experiments. “Zusanli'(ST 36) and “Neiting'(ST 44) were punctured and stimulated electrically (10?Hz, 1.5?V, continuous waves) for 10?min , once daily, continuously for 14 times. Changes of body weight, Lee's index[3body weight (g)/ body length(cm)×10 3], body fat weight, as well as the contents of β endorphin (β EP), α melanocyte stimulating hormone (α MSH), bombesin (BOM), neuropeptide Y (NPY), angiotensin Ⅱ (AngⅡ), atrial natriuretic polypeptide (ANP) and calcitonin gene related peptide (CGRP) in the hypothalamic tissue of obesity rats were assayed with radioimmunoassay. Results: The body weight, Lee's index, body fat weight, and the levels of β EP, NPY and AngⅡ in hypothalamic tissue of obesity rats were significantly higher than those of normal rats ( P <0.05～ 0.01) , but the levels of α MSH, BOM, ANP and CGRP of obesity rats were significantly lower than those of normal rats ( P <0.05～0.01). There were positive interrelation between the contents of β EP, NPY, AngⅡ and body weight, obesity index and the body fat weight; but the interrelation between the contents of α MSH, BOM, ANP and CGRP and the obesity index was negative. After acupuncture treatment the marked weight lose effect was achieved while the contents of β EP, NPY, AngⅡ in hypothalamic tissue were markedly decreased ( P <0.05～0.01), and those of α MSH, BOM, ANP and CGRP in hypothalamic tissue obviously increased ( P <0.05～0.01). Conclusion: The abnormal increase of β EP, NPY and AngⅡ levels as well as the abnormal decrease of α MSH, BOM, ANP and CGRP levels in rat hypothalamic tissue may be responsible for obesity. Acupuncture induced changes of neuropeptide levels in the hypothalamic tissue of obesity rats possibly contribute to its antiobesity action in clinic.