督脉腧穴电针对佐剂性关节炎大鼠IL-2影响的实验观察

Effect of Electroacupuncture on IL-2 Levels of Serum and Brain Tissues in Adjuvant Arthritis Rats

目的 :观察督脉腧穴电针对大鼠炎症和免疫功能的影响。方法 :制作大鼠佐剂性关节炎模型 ,观察电针“大椎”、“命门”对关节炎症局部 ,下丘脑、脾脏、肾上腺、血清白细胞介素 2 (IL 2 )的影响。结果 :电针组大鼠的局部炎症肿胀明显好转 ,且IL 2在下丘脑、脾脏、血清中的含量或活性均有改变 ,其中在下丘脑表现为降低 ,在脾脏、血清中表现为升高 (P <0 .0 1 )。结论 :电针“大椎”或“命门”能在神经、免疫、内分泌各不同水平影响IL 2的含量 ,以发挥其抗炎免疫的调节作用。同时也说明针刺督脉腧穴可以通过IL 2等共同介质对神经内分泌免疫网络进行免疫调节。

Objective: To observe the effect of electroacupuncture (EA) on the inflammation and immune function in arthritis rats. Methods: A total of 32 Wistar rats were evenly randomized into control, model, EA GV 14 and EA GV 4 groups. Arthritis model was established by subcutaneous injection of Freund's complete adjuvant into the right hind paw (0.1 mL/animal). In EA GV 14 and EA GV 4 groups, “Dazhui” (GV 14) and “Mingmen” (GV 4) were punctured and stimulated electrically (15 Hz, 1 mA) for 20 min. The treatment was given once every other day, continuously for 8 times. The foot swelling rate[( foot volume after establishing arthritis model minus foot volume before establishing model) ×100%] was calculated. IL 2 activity of spleen and IL 2 concentrations of serum and brain tissues were assayed with MTT colorimetric analysis and radioimmunoassay separately. Results: After establishing arthritis model, the foot swelling rates of both hind limbs were significantly higher than those of normal control group ( P <0.01); and following EA of both GV 14 and GV 4, the swelling rates of the left and right feet in EA GV 14 and EA GV 4 groups both decreased considerably compared with those of model group ( P <0.01), suggesting a good anti inflammatory effect of EA. No significant difference was found between EA GV 14 and EA GV 4 groups ( P >0.05) in the foot swelling rate. In arthritis rats of model group, IL 2 activity of the spleen tissue declined significantly ( P <0.01), IL 2 level in the hypothalamus tissue increased significantly ( P <0.05), no significant changes were found in IL 2 levels of the serum and adrenal gland. Compared with model group, spleen IL 2 activity of the two EA groups increased significantly ( P <0.01), hypothalamic IL 2 contents of EA groups lowered to a certain degree. Conclusion: EA has an apparent anti inflammatory effect and its resultant changes of IL 2 activity of the spleen and IL 2 content in the hypothalamus may contribute to its regulatory effect on the immune function by way of the neuroendocrine network.