摘要
目的 探讨HLA DR基因单倍型在中国南方汉族肺结核发病机制中的可能作用。方法 采用病例 对照的研究方法 ,应用PCR SSP技术对 110例中国南方汉族肺结核患者和 10 1例中国南方汉族健康对照者的 2 3个HLA DR等位基因进行分型 ,比较两组间DR5 3 DRB1、DR5 2 DRB1和DR5 1 DRB1单倍型频率 (HF)并计算其相对危险性 (RR)。结果 DR16 DR5 2单倍型的频率肺结核病例组显著高于对照组 (0 .0 1<P <0 .0 5 ) ,其RR为 3.10 ;DR1 DR5 3、DR1 DR5 2、DR13.3 DR5 3、DR12 DR5 3、DR13.3 DR5 2、DR7 DR5 2、DR9 DR5 2单倍型的频率肺结核病例组显著低于对照组 (前四者P <0 .0 1,后三者 0 .0 1<P <0 .0 5 ) ,其RR分别为 0 .2 9、0 .2 6、0 .38、0 .5 2、0 .2 5、0 .10、0 .4 2。结论 DR12 DR5 3、DR7 DR5 2、DR9 DR5 2单倍型的存在可能与中国南方汉族肺结核的发病有关联 ,而其它单倍型的差异则可能是因其组成基因的基因频率差异所致。
Objective To study possible role of HLA-DR gene haplotype in incidence of pulmonary tuberculosis among Han nationality of southern Chinese.Methods Case-control study was implemented and haplotype frequencies(HF) of DR53-DRB1?DR52-DRB1 and DR51-DRB1 were compared and relative risks (RR)were calculated by using PCR-SSP to detect 23 alleles of HLA-DR gene sites of 110 tuberculosis cases and 101 controls who come from Guangdong, Guangxi, Hunan, Hubei,Jiangxi and Fujian.Results Frequency of DR16-DR52 haplotype in pulmonary tuberculosis cases is strikingly higher than that in controls (0.01< P <0.05) and its RR is 3.10.Frequencies of DR1-DR53?DR1-DR52?DR13.3-DR53?DR12-DR53?DR13.3-DR52? DR7-DR52?DR9-DR52 haplotype in pulmonary tuberculosis cases is remarkably lower than that in controls (for DR1-DR53?DR1-DR52?DR13.3-DR53?DR12-DR53, P <0.01, for DR13.3-DR52? DR7-DR52?DR9-DR52, 0.01< P <0.05) and their RRs are 0.29?0.26?0.38?0.52?0.25?0.10 and 0.42 respectively.Conclusion DR12-DR53?DR7-DR52?DR9-DR52 haplotype are potential relative to incidence of pulmonary tuberculosis among Han nationality of southern Chinese. However, difference of frequencies of DR16-DR52?DR1-DR53?DR1-DR52?DR13.3-DR53?DR13.3-DR52 haplotype between pulmonary tuberculosis cases and controls may be caused by remarkable differences of gene frequency.
出处
《中国防痨杂志》
CAS
北大核心
2004年第1期16-20,共5页
Chinese Journal of Antituberculosis
基金
广州市科委资助项目 (2 0 0 1-Z -0 65 -1-8)