整合素与胶质瘤细胞凋亡

The Studies of Integrin and Apoptosis of Human Glioma Cell Line

目的 研究整合素 β1在胶质瘤细胞中的表达及在胶质瘤细胞增殖、生存方面的作用。 方法 流式细胞仪检测SHG4 4胶质瘤细胞整合素 β1的含量 ,以不同浓度的抗整合素 β1抗体处理在胶原蛋白I上培养的SHG4 4胶质瘤细胞 ,MTT法测定ID50 ,测定细胞生长曲线 ,流式细胞仪分析细胞周期改变。结果 流式细胞仪检测SHG4 4胶质瘤细胞表达整合素 β1,MTT曲线测定发现 ,2 0 μg/ml抗整合素 β1抗体处理SHG4 4胶质瘤细胞 4 8小时后 ,显著抑制SHG4 4胶质瘤细胞的增殖。流式细胞仪分析可检出凋亡峰。结论 整合素 β1在SHG4 4胶质瘤细胞中高表达 ,并与胶质瘤细胞增殖、生存密切相关 ,抑制整合素 β1的表达可促进胶质瘤细胞的凋亡及坏死。

Objective To investigate the expression of integrin β1 subunit and regulation of survival and proliferation in human glioma cell lines.Methods Flow cytometry detect the expression of integrin β1 subunit in SHG44 glioma cell line.Glioma cell line covered with collagen I was treated with the antibody of integrin β1 subunit at different concentrations and cell growth was analyzed by MTT assay. Cell apoptosis was evaluated by flow cytometry.Results Flow cytometry assays the high expression of integrin β1 subunit in SHG44 glioma cell line.Treatment with the antibody of integrin β1 subunit at 20μg/ml remarkably inhibited tumor cell growth and induce apoptosis.Conclusion Integrinβ1 subunit expresses in SHG44 glioma cell line and play an important role in glioma cell survival and proliferation. Inhibiting integrin β1 subunit expression can accelerate the apoptosis and necrosis of glioma cell.