环氧合酶-2在视网膜母细胞瘤中的表达

A Study of Expression of Cyclooxygenase-2 in Retinoblastoma

目的 探讨环氧合酶 2 (COX 2 )在视网膜母细胞瘤 (retinoblastoma ,RB)中的表达及COX 2选择性抑制剂对视网膜母细胞瘤细胞的作用机制。方法 应用链霉亲和素蛋白 生物素酶标免疫组织化学方法 (labellstreptavidinbiotinmethod ,LSAB) ,对 37例RB组织中COX 2的表达进行分析。培养视网膜母细胞瘤细胞HXO RB44,检测COX 2选择性抑制剂NS 398对细胞增殖的影响。结果 COX 2在RB中的阳性表达率为 75 .7% (2 8/ 37例 ) ,与RB的分化程度和视神经浸润无关 (P >0 .0 5 ) ;NS 398对HXO RB44的抑制作用具有时间及剂量依存性。结论 COX 2可能在视网膜母细胞瘤的发生和发展中起重要作用 ,COX 2选择性抑制剂NS 398对视网膜母细胞瘤细胞HXO RB44有明显抑制作用。

Objective To investigate the expression of cyclooxygenase -2 in retinoblastoma and the mechanism of COX-2's selective inhibitor,NS -398,on HXO -RB_ 44 .Methods The expression of COX-2 in RB tissues of 37 cases was analyzed by using LSAB immunohistochemical method. HXO -RB_ 44 cells were cultured.The cell growth rates were assessed,when treated with NS -398,by using MTT method.Results COX-2 was overexpressed in retinoblastoma ( 75.7% ). NS -398 inhibited the proliferation of cells in a dose and time-dependent manner.Conclusion The over expression of COX-2 is significantly related with the tumor formation of RB. HXO-RB44 cells can be inhibited by NS -398 with dose-dependent.