天年饮对亚急性衰老大鼠睾丸生精细胞增殖与凋亡的影响

The effect of Tiannianyin on proliferation and apoptosis of germ cell in testes of sub-acutely aging rats

目的 观察天年饮对亚急性衰老大鼠血清超氧化物歧化酶 (SOD)活性、睾酮含量及睾丸生精细胞增殖与凋亡的影响。方法 D-半乳糖连续腹腔注射制作亚急性衰老大鼠模型 ,检测各组大鼠天年饮灌胃前后血清 SOD的活性、睾酮的含量以及睾丸生精细胞增殖和凋亡的情况。结果 D-半乳糖致亚急性衰老大鼠血清 SOD活性、睾酮含量及睾丸生精细胞 PCNA阳性表达率均明显下降 ,与正常组比较差异显著 (P<0 .0 1 ,P<0 .0 5) ;经天年饮灌胃后 ,三者均有明显提高。 D-半乳糖致亚急性衰老大鼠睾丸生精小管凋亡百分率及凋亡阳性细胞率均增高 ,与正常组比较差异显著 (P<0 .0 5) ;经天年饮灌胃后 ,二者均降低。结论 天年饮可显著提高亚急性衰老大鼠血清 SOD活性、睾酮含量及睾丸生精细胞 PCNA的表达水平 ,降低睾丸生精细胞的凋亡指数 ,因此 ,天年饮具有延缓性腺衰老的作用。

Objective To study the effect of Tiannianyin (TNY) on the activity of superoxide dismutase (SOD) and testosterone level in serum, the PCNA expression and apoptosis of the germ cells in testes of sub-acutely aging rats. Methods Injecting D-gal into abdominal cavity continually made the models of sub-acutely aging rats. Then the SOD and testosterone levels in serum were detected, the PCNA expression and apoptosis of the germ cells in testes were observed before and after treatment with TNY. Results The SOD and testosterone levels in serum, PCNA expression of the germ cells decreased significantly in sub-acutely aging rats compared with normal rats (p<0.01, p<0.05), the three indexes increased after the treatment with THY. Apoptosis cells in sub-acutely aging rats increased significantly compared with normal rats (p<0.05) but decreased after TNY treatment. Conclusions THY has anti-aging effect on sex gland induced by D-gal in rats significantly..