环氧化酶抑制剂干预心肌缺血损伤的实验研究

Effects of cyclooxygenase inhibitor on acute ischemic myocardium in rabbits

目的 :评价环氧化酶抑制剂对心肌缺血损伤 (AIM)的保护作用。方法 :将成年家兔 (1.8～ 2 .5 kg) 2 4只随机分为正常对照组 (A组 ) ,单纯心肌缺血组 (B组 ) ,心肌缺血加迪克乐克组 (C组 ) ,建立 AIM模型 ,给予相应的干预 ,术后 6 h观察血液学指标 ,缺血心肌的 HE染色及 COX- 2的多克隆抗体免疫组织化学染色进行病理观察。结果 :缺血心肌中 COX- 2高度表达 ,血液学指标示术后 6 h白细胞计数在 B、C组间有明显差异 (P<0 .0 5 ) ,i NOS测定值在A、B组有显著差异 (P<0 .0 5 ) ,MDA、SOD含量在 A、B组间有显著性差异 (P<0 .0 5 ) ,C组应用非选择性环氧化酶抑制剂 ,在术后 6 h白细胞计数升高程度与 A、B组均有显著性差异 (P<0 .0 5 ) ,i NOS测定值与 B组无显著差异(P>0 .0 5 ) ,MDA、SOD含量与 B组差异显著 (P<0 .0 5 )。结论 :缺血心肌存在显著的炎症活动 ,环氧化酶抑制剂对实验性心肌缺血损伤有保护作用。

AIM: To evaluate the effects of cyclooxygenase inhibitor on acute ischemic myocardium(AIM) in rabbits. METHODS: 24 rabbits were randomly divided into 3 groups: group A: normal controls; group B: myocardial ischemia; group C: myocardial ischemia+diclofenac sodium sustained release tablets. The left circumflex arteries of rabbits were ligated to obtain the animal models of AIM for 6 hours in groups B and C. 6 hours after the ligation, we measured the counts of leukocyte of periphery blood and serum MDA,SOD and iNOS;HE and monoclonal antibody immunohistochemistry staining, of ischemic tissues were investigated. RESULTS: The expression of cox-2 in ischemic myocardium increased. 6 hours after the ligation, the difference in the increased count of leukocyte between groups B and C was significant(P<0.05); iNOS was similar in groups B and C(P>0.05). The differences in MDA and SOD were between groups B and C significant(P<0.05). CONCLUSION: Cyclooxygenase inhibitor can protect the ischemic myocardium.