AgNORs计数DNA含量及PCNA与肝硬化增生结节和肝癌的关系

Correlation of AgNORs, DNA contents and PCNA expression with liver cirrhosis, hyperplastic nodules and hepatocellular carcinoma

目的:探讨肝硬化(LC)、增生结节与肝细胞癌(HCC)之间的关系. 方法:分别应用银染色技术、图像分析技术及免疫组织化学技术检测LC、增生结节及HCC中AgNORs计数、DNA 含量及增生细胞核抗原(PCNA)的表达. 结果:增生结节中,其AgNORs计数、DNA含量及.PCNA 的表达均与正常肝组织和LC组织有明显差异(P分别<0.01, 0.05,0.05);其中AgNORs计数与I级HCC相近(P>0.05), DNA含量与HCC相近(P>0.05).LC组织和正常肝组织间的AgNORs计数、DNA含量及PCNA的表达差异均无显著性(P均>0.05). 结论:增生结节与LC是两种不同性质的细胞群体,前者属于活跃增生性病变,是HCC的癌前期病变,后者仍为成熟的细胞,与HCC的发生没有直接关系.

AIM: To study the relationship between liver cirrhosis (LC), liver hyperplastic nodules (LHN) and hepatocellular carcinoma (HCC). METHODS: Silver colloid, image analysis and immunohis-tochemical technique were used to examine AgNORs counts, DNA contents and the expression of proliferating cell nuclear antigen (PCNA) in LC, LHN and HCC. RESULTS: In LHN, the AgNORs counts, DNA contents and the expression of PCNA were significantly higher than those in the normal liver and LC (P<0.01, P <0.05, P<0.05, respectively); the AgNORs counts approached those in HCC grade I (P>0.05), and the DNA contents approached those in HCC (P>0.05). There was no significant difference of AgNORs counts, DNA contents and the expression of PCNA between LC and the normal liver tissues. CONCLUSION: LHN and LC are two different cell population with various characteristics; LHN is actively proliferative lesions and should be considered as a preneoplastic lesion of HCC, while LC represents mature liver cells and does not contribute directly to the hepatocarcinogenesis.