胆囊癌组织hPTTG1和bFGF表达与血管生成的关系

Expression of hPTTG1 and bFGF in gallbladder carcinoma tissue and their correlation with angiogenesis

目的:通过检测胆囊癌组织中hPTTG1、bFGF的表达和胆囊癌组织血管生成状况,探讨他们之间的相互关系及其与胆囊癌临床病理特征之间的关系. 方法:用免疫组织化学SP法对41例胆囊癌和22例慢性胆囊炎组织中hPTTG1和bFGF的表达进行检测,并用抗CD34. 抗体检测微血管密度(MVD).用MVD反映血管生成隋况. 结果:原发胆囊癌组织中hPTTG1和bFGF的表达阳性率分别为82.9%和75.6%,均高于在慢性胆囊炎中的表达阳性率(P=0.002,0.006).hPTTG1的表达与临床分期和淋巴结转移有关(P=0.025,0.007),而与组织学分级无显著关系(P=0.114);bFGF的表达与组织学分级、临床分期有关(P=0.015,0.019),而与淋巴结转移无显著关系(P=0.081); hPTTG1的表达与bFGF的表达密切相关(r=0.648,P=0.000). 二者的表达与患者的性别、年龄、肿瘤的种类,是否伴有胆囊结石均无关.胆囊癌组织MVD值明显大于慢性胆囊炎组织(P=0.001).MVD分别与胆囊癌组织hPTTG1的表达及bFGF的表达有关(P=0.000,0.000).MVD与胆囊癌组织Nevin分期及淋巴结转移有关(P=0.007,0.024);而与患者的性别、年龄、肿瘤的种类、分化程度、是否伴有胆囊结石均无关. 结论:hPTTG1的异常表达与胆囊癌的发生、发展过程及血管生成过程密切相关,可能为胆囊癌的诊治提供了一条新的途径.

AIM: To investigate the expression of hPTTGl and bFGF in human gallbladder carcinoma tissues and their correlation with angiogenesis and other clinicobiological behaviors. METHODS: The expression of hPTTGl and bFGF in 41 cases of human gallbladder carcinoma and 22 cases of chronic cholecystitis was detected by immunohistochemical staining (SP method). The microvessels were highlighted by immunohistochemical staining (SP method) to detect antigen of CD34. Angiogenesis was represented by intratumor microvessel density (MVD). RESULTS: In the gallbladder carcinoma, the positive rates of hPTTGl and bFGF were 82.9% and 75.6% respectively, which were significantly higher than those in the chronic cholecystitis (P =0.002 and 0.006). The expression of hPTTGl was significantly associated with clinical stages and lymph node metastasis status (P =0.025, 0.007), but not with histological differentiation (P=0.144). The expression of bFGF was significantly correlated with clinical stages and histological differentiation (P =0.019, 0.015), but not with lymph node metastasis status (P =0.081). There was a significant correlation between the expression of hPTTGl and bFGF (r =0.648, P =0.000). Neither of them had relation with age, sex, histological type and cholelithiasis. The value of MVD in the gallbladder carcinoma was significantly higher than that in the chronic cholecystitis (t =3.684, P =0.001). The expression of hPTTGl and bFGF was correlated with MVD in gallbladder carcinoma (P =0.000, 0.000). MVD in the gallbladder carcinoma was significantly associated with clinical stages and lymph node metastasis status (P =0.007, 0.024), but not with age, sex, histological type, histological differentiation and cholelithiasis. CONCLUSION: Overexpression of hPTTGl is related to the tumorigenesis and angiogenesis in gallbladder carcinoma,which may provide a new target for therapy of gallbladdercarcinoma.