脾气虚证患者血浆和组织中SS、Mot、CCK的变化

Changs of somatostatin, motilin andcholecystokinin in gastric antrum tissue and plasma in patients with spleenasthenia

目的:通过观察四君子汤对脾气虚证患者血浆和胃窦黏膜组织中胃动素(Mot)、生长抑素(SS)、胆囊收缩素(CCK)的影响,探讨脾气虚证临床辨证的客观定量指标. 方法:用放射免疫分析法(RIA)动态观察服药前后脾气虚证和胃热炽盛患者血浆和胃窦黏膜组织中Mot、SS和CCK的变化. 结果:(a)脾气虚证患者组织及血浆中Mot水平服药前显著低于胃热炽盛组及服药后,(胃窦组织:109.4±18.9 ng/g vs 219.8±25.3ng/g及183.0±23.9ng/g;血浆:61.7±10.6ng/L vs 171.4±30.8 ng/L及126.3±24.6 ng/L)(P<0.01);(b)脾气虚证患者组织和血浆中SS含量服药前显著高于胃热炽盛组及服药后,(胃窦黏膜:224.3±67.9 ng/g vs 80.2±25.6 ng/g 及114.2±34.5ng/g;血浆:74.5±17.2 ng/L vs30.3±11.8ng/L 及38.1±12.7 ng/L)(P<0.01);(c)脾气虚证患者组织和血浆中CCK含量服药前显著高于胃热炽盛组及服药后,(胃窦黏膜组织:34.1±5.2 ng/g vs 21.6±4.7 ng/g及29.8±6.8 ng/g;血浆:5.8±0.4 ng/L vs 3.7±0.6 ng/L及2.9±0.8 ng/L)(P<0.01);(d)胃热炽盛组在服药后Mot、SS和CCK在血浆和组织中的含量变化不明显(P>0.05)(数字略). 结论:Mot、SS和CCK的浓度变化对脾气虚证的临床辨证施治可能有重要的参考价值.

AIM: To explore the objective quantitative index of spleen-asthenia by investigating the effects of Sijunzhi decoction on the somatostatin, motilin and cholecystokinin in gastric antrum tissues and plasma in patients with spleen-asthenia. METHODS: The levels of somatostatin (SS), motilin (Mot) and cholecystokinin (CCK) in gastric antrum tissues and plasma in patients with spleen-asthenia and stomach-heat were observed before and after treatment by RIA, respectively. RESULTS: Compared with the stomach-heat and the spleen-asthenia treated by Sijunzhi decoction, patients with spleen-asthenia before the treatment had significantly higher level of motilin both in the tissues and plasma (109.4±18.9 ng/g vs 219.8±25.3 ng/g, and 183.0±23.9 ng/g;plasma: 61.7±10.6 ng/L vs 171.4±30.8 ng/L, and 126.3±24.6 ng/L) (P<0.01); The SS contents in the tissues and plasma were increased 'antrum' (224.3±67.9 ng/g vs 80.2±25.6 ng/g, and 114.2± 34.5 ng/g; plasma: 74.5±17.2 ng/L vs 30.3±11.8 ng/L, and 38.1±12.7 ng/L) (P<0.01): The CCK levels in gastric antrum and plasma were also arisen (tissues: 34.1±5.2 ng/g vs 21.6±4.7 ng/g, and 29.8± 6.8 ng/g; plasma: 5.8±0.4 ng/L vs 3.7±0.6 ng/L, and 2.9±0.8 ng/L)(P <0.01). The con- tents of Mot, SS and CCK in patients with stomach-heat were not significantly changed (P >0.05). CONCLUSION: The contents of Mot, SS and CCK may have important values in the clinical diagnosis and treament for patients with spleen-asthenia.