桃红四物汤促进实验性骨折愈合的分子机理研究

Study on the Molecule Mechanism of Taohongsiwu Decoction Promoting the Healthing of Experimental Fracture

目的 研究桃红四物汤对骨折断端骨痂组织内VEGF表达及对骨折愈合的影响。方法 将日本大耳白兔65只随机分成4组,3组分别行右侧桡骨中段手术骨折造模后给予桃红四物汤、炎迪宁片、蒸馏水灌胃,另1组为正常对照组,在骨折后7、15、21、35d摄取骨折部位X线片,同时取骨折断端标本脱钙,采用免疫组化方法检测骨痂组织中VEGF表达,并作计量对比分析。结果 桃红四物汤干预组VEGF表达量高于其它各组,并在第15天表达峰值后下降不明显,同时使骨折局部血肿吸收加快,骨痂生长和塑形优于其它各组。结论 桃红四物汤能提高骨折后骨痂中VEGF的表达,加速断端血肿吸收和骨痂生长塑形,促进骨折愈合。

Objective To study the effect of Taohongsiwu Decoction (THSWD) to the expression level of VEGF of callus tissue in the bone stump and the healthing of fracture. Methods 65 Japanese rabbits were divided into four groups randomly, three groups were given orally with THSWD, Yandining tablet, distilled water respectively after the right radial bone fracture model was established by operation, the other group was normal group. At the 7th, 15th, 21th and 35th day after fracture, we took the X-ray film on the extremities of fracture and decalified the bone stumps as spicemen in every group above, the methods of immunohischemistry were used to detect the expression level of VEGF in the callus tissue of bone stump, then we compared and analyzed the data by measurement. Result The expression of VEGF in THSWD invervening group was higher than other groups. THSWD could make the decrease of expression was not obvious after the peak value at the 15th day, it could accelerate the blood swelling absorption and make the growth and remodeling of the callus are better than other groups. Conclusion THSWD can could improve the expression level of VEGF of callus tissue in bone stump, accelerate blooding swelling absorption and promote the growth and remodeling of the callus, promote the healthing of fracture.