云南赤芍抗乙型肝炎病毒的体外实验研究

<title>perimental Study of Anti-HBV Effects of Radix Paeoniae Rubra in Yunnan in vitro

目的:初步研究云南赤芍的抗乙型肝炎病毒(HBV)作用。方法:通过云南赤芍作用于体外培养的2.2.15细胞,观察其对2.2.15细胞两抗原分泌的影响.初步评价其抗HBV作用。结果:云南赤芍作用于2.2.15细胞12d后,药物对2.2.15细胞的半数毒性浓度为3.85g／L,对HBsAg、HBeAg的半数抑制浓度分别为<0.156g／L、0.54g／L,治疗指数分别为>24.68、7.13。结论:云南赤芍在体外细胞培养中对两抗原的分泌有较好的抑制作用。

<abstract>jective:To explore anti-HBV effects of Radix Paeoniae Rubra in Yunnan (RPRY). Methods: 2. 2. 15 cell line was taken as cell model. The influence of RPRY on the secretion of the two antigens of 2. 2. 15 cells was observed. Results:Twelve days after the action of RPRY on the cells, its LD50 was 3. 85 g/L;ID50 to the HBsAg and HBeAg was lower than 0. 156 g/L and 0. 54 g/L respectively, Treatment index was higher than 24. 68 and 7. 13 respectively. Conclusion:RPRY can markedly inhibit the secretion of the two antigens in 2. 2. 15 cell line.