牛IVF性控切割胚胎和整胚的冷冻保存研究

Cryopreservation of biopsied and intact bovine IVF blastocysts

本研究分 3个实验对比了采用不同处理的常规冷冻和玻璃化冷冻保存 3 5 8枚体外受精 (IVF)性控切割囊胚和 3 2 6枚整胚的保存效果。结果表明 :(1 )用于冷冻整胚的常规方法对冷冻性控切割胚胎同样有效 ;(2 )利用含 1 0 % EG +1 0 % FBS的 DPBS冷冻牛 IVF性控切割胚胎或整胚的效果明显优于用含1 0 %甘油 +0 .4% BSA或 1 .4mol/ LEG+0 .1 % PVOH的 DMPBS;(3 )采用微玻管玻璃化冷冻保存牛 IVF性控切割胚胎的效果显著优于常规冷冻法。

Bovine biopsied ( n =358) and intact ( n =326) in vitro fertilization(IVF) blastocysts were frozen by different conventional freezing methods or vitrified by a micro-straw method.The results showed:(1) The same efficiency (survival and quality after thawing) was obtained after freezing biopsied compared to intact blastocysts using conventional freezing methods.(2) Both biopsied and intact bovine blastocysts frozen in 10% EG in DPBS supplemented with 10% FBS achieved much higher survival and usability rates compared to freezing in 10% glycerol + 0.4% BSA or 1.4 mol/L EG + 0.1% PVOH in DMPBS. (3) The rates of survival and usability of vitrified biopsied blastocysts were significantly higher than that of conventionally frozen blastocysts when evaluated 20 h after thawing.