生物瓣材料体外人工内皮化的实验研究

Experimental Study on Chemical Modification and Endothelialization of Bioprosthetic Heart Valve Materials

目的 :探索一种有效的生物瓣材料内皮化方法 ,延长生物瓣的使用寿命。方法 :新鲜牛心包片分三组处理 :Ⅰ组单纯戊二醛 (GA)处理 ;Ⅱ组GA +2 ,3 丁二醇改性 ;Ⅲ组GA +2 ,3 丁二醇改性 +Ⅰ型胶原蛋白预覆 ;空白玻片作对照 (Ⅳ组 )。各组体外种植内皮细胞 ,计算种植后第 1、3、5、8d的活细胞数。种植后第 8d行第Ⅷ因子检测和扫描电镜 (SEM )观察。结果 :Ⅲ组牛心包片表面细胞生长最为活跃 ,在第 5、8d的细胞数明显多于Ⅰ组和Ⅱ组 (P<0 .0 5 ) ,细胞与基质间有微丝连接形成。结论 :2 ,3 丁二醇改性和预覆Ⅰ型胶原蛋白是生物瓣材料体外人工内皮化的有效和可行的方法 ,具有潜在的临床应用前景。

Objective: To study an effective way for endothelialization of bioprosthetic heart valve material to improve its durability. Methods: Fresh bovine pericardiums (BPs) were divided into three groups: groupⅠ treated with glutaraldehyde;groupⅡ modified with the 2,3-butanediol;groupⅢ modified with the 2,3 butanediol and precoated with collagen type. The glass plates were used as control (group Ⅳ). Endothelial cells (ECs) were seeded onto the surfaces of BPs,and counted on day 1,3,5 and 8,and identified by factor Ⅷ staining and evaluated by scanning electron microscopy (SEM) on 8th day after seeded. Results: On day 5 and 8,the amount of ECs in groupⅢ were significantly more than in groupⅠ and groupⅡ ( P <0.05). BPs of groupⅢ were similar to native heart valve. Conclutions: Endothelialization of bioprosthetic materials with the 2,3 butanediol modified and collagen typeⅠ precoated treatment in vitro is effective.It is potentially available for clinical use.