摘要
目的 :探讨含Ⅰ、Ⅱ、Ⅳ型菌毛的绿脓杆菌制剂 ,在体外对正常人外周血单个核细胞 (PBMC)的活化作用。方法 :以绿脓杆菌菌毛制剂 ,作用于体外培养的人PBMC ,采用生物素 -链霉亲和素 (BSA)免疫细胞化学法 ,检测人PBMC表面CD2、CD2 5、CD6 9的表达。结果 :该制剂在实验最适条件下 ,可使PBMC上CD2表达率增加15 2 % ,CD6 9表达率增加 2 9.1% ,CD2 5的表达率增加 18.7% ,这与对照组比较差异非常显著 (P <0 .0 1)。结论 :绿脓杆菌菌毛制剂在体外能有效地促进单个核细胞的活化 ,提示该制剂可增强机体的细胞免疫功能。
Objective:To study the effect of PPA (Piliated Pseudomonas aeruginosa strain;PPA) on expression of CD2,CD25 and CD69 of human PBMC. Methods: The techniques of immunocytochemical method and biotin streptavidin (BSA) were applied for evaluation of expression rate of CD2,CD25 and CD69 of human PBMC by PPA stimulation. Results: PPA enhanced significantly CD2,CD25 and CD69 expression as compared with the expression rate in controls. Conclusion: This result indicated that PPA can improve cellular immuno functions.
出处
《广州医学院学报》
2003年第1期46-48,共3页
Academic Journal of Guangzhou Medical College
