一种改良的免疫组织化学染色方法

An Modified Immunohistochemistry Staining Method

目的 :探讨一种新的有效检测出肺部血管内皮生长因子 (VEGF)表达的免疫组化方法。方法 :实验分三组 ,分别为阴性对照组、常规消除背景组、改良组。阴性对照组包括DAKO公司二抗组 ,华美公司二抗组。余两组均包括阴性对照组及正常组。结果 :常规消除背景组有效消除背景但无法检出肺部VEGF表达。改良组既能有效消除背景 ,又能成功检测出待测物质。结论 :改良组有效消除背景干扰 ,检测出肺部VEGF表达。

Objectives:To explore a new and more effective method to detect vascular endothelial growth factor (VEGF) expression in rat lung. Methods:The study included three groups : negative control group,normal diminishing background group,and modifided immunohistochemistry group. The negative control group compared the background caused by the secondary antibody ( Huamei company and DAKO company).The two other groups all included negative control group and normal group. Results:The background was diminished in normal diminishing background group,but VEGF expression failed to be detected. In modified way,the VEGF expression in rat lung was not only detected effectively but also without background. Conclusion:The VEGF expression in rat lung was not only detected effectively but also without background in modified way.