摘要
Objective: To research the relationship among CEA expression in tumor, CEA serum level and Radioimmunoguided Surgery (RIGS) in Colorectal Cancer. Methods: twenty-nine patients with colorectal cancer wer administered radiolabeled McAb CL58 submucosally via endoscope, and all underwent surgical intervention 3 to 14 days later. Intraoperative radioimmunodetection was performed using a portable gamma-detecting probe (GDP). The counts of tumors were obtained, and tumor-to-normal tissue (T/NT) ratio was calculated. The T/NT ratio of 3:1 was taken as the lowest threshold value of positiveness. Anti-CEA immunohistochemical for all the samples were performed. The CEA serum levels of all the patients before operations were also recorded. Results: The sensitivity for RIGS in detecting primary lesions was 93.1% (27/29). 28 patients (96.6%) had CEA expressions to a greater or less extent. 5 patients (17.2%) had elevated CEA serum. There was no correlation between the CEA serum levels and results of RIGS (P>0.5), and no correlation between the CEA serum levels and CEA expression in tumor (P>0.5). The CEA expression in tumor correlated significantly with the RIGS results (P<0.05). Conclusion: This study indicates that immunostaining can be used to select the patients as candidates who will benefit most from RIGS.
Objective: To research the relationship among CEA expression in tumor, CEA serum level and Radioimmunoguided Surgery (RIGS) in Colorectal Cancer. Methods: twenty-nine patients with colorectal cancer wer administered radiolabeled McAb CL58 submucosally via endoscope, and all underwent surgical intervention 3 to 14 days later. Intraoperative radioimmunodetection was performed using a portable gamma-detecting probe (GDP). The counts of tumors were obtained, and tumor-to-normal tissue (T/NT) ratio was calculated. The T/NT ratio of 3:1 was taken as the lowest threshold value of positiveness. Anti-CEA immunohistochemical for all the samples were performed. The CEA serum levels of all the patients before operations were also recorded. Results: The sensitivity for RIGS in detecting primary lesions was 93.1% (27/29). 28 patients (96.6%) had CEA expressions to a greater or less extent. 5 patients (17.2%) had elevated CEA serum. There was no correlation between the CEA serum levels and results of RIGS (P>0.5), and no correlation between the CEA serum levels and CEA expression in tumor (P>0.5). The CEA expression in tumor correlated significantly with the RIGS results (P<0.05). Conclusion: This study indicates that immunostaining can be used to select the patients as candidates who will benefit most from RIGS.
基金
This work was supported by the State 863 High Technology R&D Project of China (No.2001AA21537).
