HCA520, A NOVEL TUMOR ASSOCIATED ANTIGEN, INVOLVED IN CELL PROLIFERATION AND APOPTOSIS
HCA520, A NOVEL TUMOR ASSOCIATED ANTIGEN, INVOLVED IN CELL PROLIFERATION AND APOPTOSIS
摘要
Objective: Tumor associated antigen encoding gene HCA520 (AF146019) was identified by screening a human hepatocellular carcinoma expressing cDNA library using SEREX technique. In this experiment we studied the effect of HCA520 on cell proliferation and apoptosis. Methods: Gene HCA520 was gained by PCR and transfected into 293 cells. The stable expression cells were obtained by G418 selection. The cell proliferation was measured by [3H]-TdR uptake and apoptosis assay was measured by FACS. Results: Eukaryotic expression plasmid pcDNA3-HCA520 was constructed and its stable transfectants were obtained. Overexpression of HCA520 inhibited the cell proliferation and enhanced cell apoptosis after serum deprivation. Conclusion: HCA520 is a novel tumor associated antigen that can affect cell proliferation and apoptosis.
Objective: Tumor associated antigen encoding gene HCA520 (AF146019) was identified by screening a human hepatocellular carcinoma expressing cDNA library using SEREX technique. In this experiment we studied the effect of HCA520 on cell proliferation and apoptosis. Methods: Gene HCA520 was gained by PCR and transfected into 293 cells. The stable expression cells were obtained by G418 selection. The cell proliferation was measured by [3H]-TdR uptake and apoptosis assay was measured by FACS. Results: Eukaryotic expression plasmid pcDNA3-HCA520 was constructed and its stable transfectants were obtained. Overexpression of HCA520 inhibited the cell proliferation and enhanced cell apoptosis after serum deprivation. Conclusion: HCA520 is a novel tumor associated antigen that can affect cell proliferation and apoptosis.
参考文献8
-
1[1]Wang Y, Han KJ, Pang XW, et al. Large scale identification of human hepatocellular carcinoma- associated antigens by autoantibodies[J]. J Immunol 2002; 169: 1102.
-
2[2]Lin X. Barber DL. A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange[J]. Proc Natl Acad Sci USA 1996; 93:12631.
-
3[3]Pang T, Su X, Wakabayashi S, et al. Calcineurin Homologous Protein as an Essential Cofactor for Na+/H+ Exchangers[J]. J Biol Chem 2001; 276: 17367.
-
4[4]Rotin D, Steele-Norwood D, Grinstein S, et al. Requirement of the Na+/H+ exchanger for tumor growth[J]. Cancer Res 1989; 49:205.
-
5[5]Reshkin SJ, Bellizzi A, Caldeira S, et al. Na+/H+ exchanger-dependent intracellular alkalinization is an early event in malignant transformation and plays an essential role in the development of subsequent transformation-associated phenotypes[J]. FASEB J 2000; 14: 2185.
-
6[6]Pilatte Y, Vivo C, Renier A, et al. Absence of SV40 large T-antigen expression in human mesothelioma cell lines[J]. Am J Respir Cell Mol Biol 2000; 23: 788.
-
7[7]Lin X, Sikkink RA, Rusnak F et al. Inhibition of calcineurin phosphatase activity by a calcineurin B homologous protein[J]. J Biol Chem 1999; 274: 36125.
-
8[8]Pang T, Wakabayashi S, Shigekawa M. Expression of Calcineurin B homologous protein 2 protects serum deprivation-induced cell death by serum-independent activation of Na+/H+ exchanger[J]. J Biol Chem 2002; 277: 43771.
-
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-
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-
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