ICAM-1协同参与TNFRI介导的TM-TNF-α杀瘤作用

Participation of ICAM-1 in the cytotoxicity of TM-TNF-α mediated by TNF receptor I

目的 :寻找协同参与TM TNF α杀瘤作用的膜表面分子 ,探讨TM TNF α杀瘤及两型TNF α生物学效应差异的分子机制。方法 :利用抗体封闭实验及RT PCR ,检测ICAM 1及VCAM 1对TNFR1或TNFRII介导的TM TNF α杀瘤效应是否具有协同作用。结果 :封闭表达TNFRI的MCF 7细胞表面的ICAM 1,可显著降低TM TNF α对其杀伤的作用 ;而封闭VCAM 1无明显效应。封闭表达TNFRII的HL 6 0细胞表面的ICAM 1或VCAM 1,均对TM TNF α的杀伤作用无影响。结论 :ICAM 1对于TM TNF

AIM: To identify membrane-associated molecules involved in the tumoricidal cytotoxicity of TM-TNF-α and to explore the molecular mechanism underlying the tumoricidal cytotoxicity of TM-TNF-α and differences of biological effects between TM-TNF-α and sTNF. METHODS: Antibody blocking test and RT-PCR were used to evaluate the role of ICAM-1 and VCAM-1 in the cytotoxicity of TM-TNF-α mediated by TNFR I or TNFR II. RESULTS: After the ICAM-1 expressed on the membrane of MCF-7 cells(expressing TNFRI) was blocked, the cytotoxicity of TM-TNF-α to this cell lines decreased significantly, while blocking VCAM-1 had no effect. Blocking ICAM-1 or VCAM-1 expressed on the membrane of HL-60 cells(expressing TNFRII)had no significant effects on the cytotoxicity of TM-TNF-α to this cell lines. CONCLUSION: ICAM-1 participates in the cytotoxicity of TM-TNF mediated by TNFRI.