通痹灵总碱对T细胞内Th1型细胞因子表达的影响

Effects of total alkaloids of Tongbiling prescription on Th1 type cytokine expression in T lymphocytes

目的 :研究通痹灵总碱 (TBL)对T细胞中Th1型细胞因子表达的影响 ,并揭示其抗炎机制 ,为临床用药提供理论依据。方法 :分离小鼠肠系膜淋巴结细胞 ,加入不同浓度的TBL作用 1h ,再加多克隆刺激剂佛波醇酯 (PDB)和离子酶素 ,继续培养 4h收获细胞 ,进行双色荧光素标记 ,以流式细胞术对Th1型细胞因子INF γ和TNF α的表达进行分析。结果 :2 0 0mg/L 和 10 0mg/L的TBL ,能显著抑制T细胞内INF γ和TNF α的表达。结论 :TBL可抑制Th1型炎症性细胞因子的表达 。

AIM: To study the effects of total alkaloid of Tongbiling prescription(TBL) on Th1 type cytokine expression in T cells in order to elucidate the anti-inflammatory mechanism of TBL. METHODS: The lymphocytes were isolated from mouse mesenteric lymph nodes and cultured in vitro. Various concentrations of TBL were added to the culture followed by phorbol ester and inomycin treatment and then incubated for another 4 hours. The expressions of IFN-γ and TNF-α in the lymphocytes were analyzed by flow cytometry. RESULTS: 200 mg/L and 100 mg/L TBL could obviously inhibit IFN-γ and TNF-α expressions in T lymphocytes. CONCLUSION: Inhibiting Th1 cytokine expression may be one mechanism by which TBL can treat rheumatoid arthritis.