鳖血提取物对小鼠免疫功能正向调节作用的研究

Immunological up-regulation of cyclophosphamide-treated mice by turtle blood extract

目的 :研究鳖血提取物对小鼠的免疫调节功能。方法 :采用环磷酰胺为免疫抑制剂 ,获得免疫功能低下的小鼠动物模型。比较鳖血提取物作用前后 ,小鼠T细胞亚群的数量、NK细胞的杀伤功能 ,淋巴细胞的增殖功能以及细胞因子水平的变化。结果 :鳖血提取物对免疫功能低下小鼠的CD4 + T细胞在外周血中的比例、NK细胞的杀伤活性、淋巴细胞的增殖功能 ,均具有正向调节作用 (P <0 .0 5 ) ,并呈剂量依赖性 ;能提高淋巴细胞分泌IFN γ的能力。结论

AIM: To explore the effect of turtle blood extract on murine immune system. METHODS: We compared the ratio of CD4 + helper T cells and CD8 + T cells, NK cell activity, lymphocyte proliferation stimulated by mitogen and capability of cytokine secretion of cyclosphophamide-treated mice with or without treatment of turtle blood extract. RESULTS: Turtle blood extract-treated mice displayed increased CD4 + helper T cell population, stronger cytotoxicity of NK cells and enhanced of ConA-induced lymphocyte proliferation. In addition, the capacity of IFN-γ secretion was dramatically up-regulated in turtle blood extract-treated mice group. CONCLUSION: Turtle blood extract can upregulate the immune function of cyclosphophamide-treated mouse.