恶性疟原虫Pf12基因重组质粒DNA接种诱导小鼠的免疫应答

DNA Vaccine of Plasmodium falciparum:immune response of mice induced by injection of the recombinant plasmid DNA of Pf12

目的 观察重组质粒VR10 12 -Pf12DNA直接免疫接种诱导BALB/c小鼠所产生的免疫应答 ,分析Pf12基因的抗原性和作为疫苗抗原候选分子的可能性。方法 构建重组质粒VR10 12 -Pf12 ,直接免疫BALB/c小鼠 ,通过NK细胞杀伤活性、脾T淋巴细胞增殖水平、ELISA、体外抑虫试验测定 ,观察其诱导的细胞和体液应答以及体外抑虫效果。结果 重组质粒VR10 12 -Pf12免疫BALB/c小鼠 ,NK细胞杀伤活性、脾T淋巴细胞增殖水平明显高于对照组 (P <0 0 1) ,诱导小鼠产生的抗体水平明显增高 (P <0 0 1) ,免疫血清在体外能抑制恶性疟原虫的生长、发育。结论 重组质粒VR10 12 -Pf12DNA直接免疫接种诱导BALB/c小鼠产生一定水平的体液和细胞免疫应答 ,其免疫血清在体外对恶性疟原虫的生长、发育有明显的抑制作用。

Objective To observe the level of immune responses of BALB/c mice induced by direct injection of the recombinant plasmid VR1012-Pf12 and to analyze its probability as DNA vaccine of Plasmodium falciparum.Methods The recombinant plasmid VR1012-Pf12 was constructed and immuned to the mice for 3 injections.NK cell killing activity,T lymphocyte proliferation activity,ELISA,inhibitory assay of the growth and development of P.f.in vitro were used to determine of Th-cell and humoral responses.Results At 60th days after 3 injections,NK cell killing activity and proliferation activity of splenic T lymphocyte in VR1012-Pf12 group were higher than that in VR1012 group(P<0.01) and NS group(P<0.01).The value of IgG in the mice increased and the titers of IgG were higer than that in VR1012 group(P<0.01) and NS group(P<0.01).The antiserum of VR1012-Pf12 group could inhibit the growth and development of erythrocytic stages of P.f.in vitro.Conclusion Direct injection with recombinant plasmid VR1012-Pf12 could elicite BALB/c mice to generate humoral and cell -mediated immune responses,the antiserum obtained could effectively inhibit the growth and development of P.f.in vitro.