结核分枝杆菌L型的垂直感染及其对子代小鼠的影响

Vertical transmission of Mycobacterium tuberculosis L-forms and its influence on the 2nd generation mice

目的 研究结核分枝杆菌L型的垂直感染及其对子代小鼠的影响。方法 将C5 7BL/ 6N小鼠 (雌∶雄 3∶2 )随机分成 4组 ,分别用自肺癌患者血中分离的结核分枝杆菌L型 (B组 ) ,牛型分枝杆菌 (A组 )及其L型 (C组 )经尾静脉进行感染 ,并用生理盐水注射组作为对照 ,观察感染鼠子代新生鼠和子代成年鼠的病理学改变 ,同时采用培养法、IK抗酸染色和免疫组织化学技术检查组织内的抗酸杆菌及其L型。结果 感染鼠的肺、肝、肾、脑、心等主要脏器组织均发生间质性炎症 ,并有多只小鼠伴有肝脏和卵巢的瘤样增生。新生子鼠中 ,A和B组有间质性炎症表现 ,B组有一例肝瘤样增生 ,C组有一例畸胎 ,组织内有多种形态的分枝杆菌L型 ;成年子鼠中A组有两只良性畸胎瘤。结论 血源性结核分枝杆菌L型和牛型分枝杆菌L型仍有一定的致病性并可能诱导肿瘤发生。通过垂直感染 ,结核分枝杆菌L型可导致子代小鼠的病变。

Aim To study the vertical infection of M.tuberculosis L-forms and its influence on the 2nd generation mice.Methods 40 C57BL/6N mice (female∶male 3∶2) divided at random into 4 groups,were infected with M.tuberculosis L-forms in blood( group B),M.bovis in bacterial forms (group A),L-forms( group C),and with normal saline as control seperately.Vertical transmission of infection was examined in the mice of the 2nd generation.Culturation,Intensified-Kinyoun's acid-fast staining,and immunohistochemical staining were used for examination.Results We found that interstitial changes with infiltration of mononuclear macrophages and lymphocytes in lungs,liver,kidneys,brain and hearts of all directly infected groups,and neoplasm-like proliferations in liver and ovaries of some infected animals.In the new born mice,interstitial inflammations of various organs were found in group A and B,a neoplasm-like proliferation of liver in group B,and an anomaly was found in group C.Mycobacterial L-forms were observed in these sections.In adults mice of the 2nd generation,2 benign teratomas in group A were observed.Conclusion Hematogenous L-forms of M.tuberculosis from patient with lung cancer and M.bovis are still pathogenic and may lead to vertical transmission of the infection,to the fetus,they may cause tumour formation.