摘要
目的 观察重症肌无力 (myasthenia gravis,MG)患者 Ig G(ACh RAb)经大鼠脑室内注入对其脑电图 (elec-troencephalography,EEG)及脑干听觉诱发电位 (brain stem auditory evoked potential,BAEP)的影响 ,并探讨ACh RAb影响大鼠中枢神经系统 (CNS)的机制。方法 将从确诊的、ACh RAb阳性的 MG患者血清中提纯的Ig G(ACh RAb) ,注射到实验组大鼠侧脑室 ,对照组大鼠则注射健康人 Ig G,观察大鼠行为学、 BAEP、 EEG改变。结果 实验组大鼠术后出现类似于实验性自身免疫性 MG的行为学改变 ,部分大鼠出现癫痫发作 ;BAEP峰间潜伏期 (IPLs)延长 ;EEG异常 ,即δ、θ增多与痫性波发放增加。结论 MG ACh RAb可致大鼠 CNS损害 ,ACh RAb与大鼠 CNS神经元型乙酰胆碱受体结合 ,可能是其病理生理机制。
Objective To observe the effects of nicotinic acetylcholine receptor antibody (AChRAb) on rats brainstem auditory evoked potential (BAEP) and electroencephalography(EEG) as well as mechanism. Methods IgG (containing AChRAb) purified from myasthenia gravis patients sera was injected to the models lateral ventricle, and the same injections were performed on the control with the IgG derived from healthy volunteers. The rats BAEP and EEG were examined one day before and two weeks after the injections.Results While after the injections, the model rats showed abnormal behavior, as well as abnormal BAEP and EEG were observed. Conclusions The results suggested that AChRAb may cause rats CNS dysfunction.
出处
《中国神经免疫学和神经病学杂志》
CAS
2004年第1期9-12,共4页
Chinese Journal of Neuroimmunology and Neurology
基金
广西科学基本项目基金资助项目 (0 2 3 60 2 4)
