自制铜圈栓塞兔髂动脉及肾动脉的实验研究

Experimental study of embolization by self-made copper coils in rabbits′ iliac and renal arteries

目的 探讨自制铜圈对兔髂、肾动脉的栓塞作用。方法 自制铜圈、弹簧钢圈、丝线分置体外抗凝血皿中 ,观察其促凝血性。 2 0只兔一侧髂、肾动脉用铜圈栓塞 ,另侧髂动脉用医用钢丝栓塞作对照 ,术后不同时间观察血管闭塞情况及其病理学改变 ;测肝、肾功能及血清铜离子浓度。结果体外实验示铜圈有较强的促凝血作用。血管造影示铜圈闭塞动脉效果优于钢丝 ,差异有非常显著性意义 (P <0 0 1)。病理检查表明铜圈能早期诱发血栓且较钢丝明显差异有非常显著性意义 (P <0 0 5 ) ;铜圈对周围组织的影响与钢丝对比不明显 ,差异无显著性意义 (P >0 0 5 )。血液铜离子术后3d较术前升高 ,差异有显著性意义 (P <0 0 5 ) ,1周后与栓前比较 ,差异无显著性意义 (P >0 0 5 ) ;肝、肾功能 2周后恢复至术前水平。结论 自制铜圈对活体兔髂、肾动脉栓塞效果好 。

Objective To evaluate the embolic effect of self made copper coil in rabbits′ iliac and renal arteries. Methods In vitro study, the blood coagulative ability of the copper coil, steel coils, and silk thread were observed at 12 h and 24 h, respectively. In animal experiment, 20 rabbits were used, and either right or left renal and iliac arteries were embolized by using the copper coils. As the control, the iliac arteries on the unexperimented side were embolized by using the steel coils. After the embolization, the arteriography, the level of serum copper ions, liver and renal functions, and the tissue histology were respectively evaluated at different time intervals. Results In vitro study, the copper coil had stronger blood coagulative ability than others ( P <0.01). In animal experiment, the ability of artery occlusion and accelerative thrombosis by copper coils were more effective than that of the control groups ( P <0.01). After the embolization, the level of serum copper ions was increased at the 3rd day ( P <0.05), but recovered to preoperative level at 1 week ( P >0.05), and the liver and renal functions were similar to that of the preoperation after 2 weeks. Conclusion The self made copper coil has a good embolic effect in rabbits′ iliac and renal arteries, and it has no significant side and toxic effects.