麻醉对小鼠热休克反应的影响

EFFECTS OF ANAESTHESIA ON THE HEAT SHOCK RESPONSE OF MICE

目的 通过分析麻醉状态和清醒状态小鼠在相同热休克条件下的热休克反应异同 ,了解整体调控在生物热休克反应中的意义和作用。 方法 经 4 0、4 2、4 4和 4 6℃ ,分别处理清醒状态和麻醉状态小鼠 30min ,于正常饲养条件下恢复 2 4h后 ,检测其肝脏热休克蛋白 70 (HSP70 )的表达差异及酸性和中性蛋白水解酶活性变化。 结果 当热休克温度为 4 4～ 4 6℃时 ,清醒状态小鼠肝脏的HSP70合成能力下降 ,而麻醉小鼠肝脏保持较强的HSP70合成能力 ;两组小鼠肝脏的酸性蛋白水解酶活性基本与HSP70合成正相关 ,中性蛋白水解酶活性基本与HSP70合成负相关。 结论 麻醉状态小鼠肝脏的热休克耐受性大于清醒小鼠 ;小鼠肝脏的热休克反应受整体和细胞两个水平调控 。

Objective In order to understand and evaluate the significance and action of the whole body's regulation in the organism's heat shock response, this paper analyzed the difference between the anaesthetic and sober mice in the same heat shock condition. Methods The anaesthetic and sober mice were treated respectively in 40, 42,44 and 46℃ for 30?min and then recovered for 24?h in normal raise. The heat shock protein 70(HSP70) in mouse livers were checked by Western blot.The acid and neutral proteinase activities in mouse livers were checked by renatured electrophoresis of proteinases. Results HSP70 synthsis in the sober mouse livers was decreased by 44\|46℃, but it was stronger in the anaesthetic mouse livers. The acid proteinase activities in the sober and anaesthetic mouse livers basically indicated a positive relationship with HSP70 synthesis, but the neutral proteinase activities showed a negative relationship.Conclusion Based on the results, it was inferred that the anaesthetic mouse livers had a higher heat\|resistance than that of the sober mouse livers, and that the stress response of the mouse livers was regulated by two levels of cells and whole body, which also involved in other physiological and biochemical actions except the heat shock protein synthesis. [