摘要
目的 探讨血红素加氧酶 一氧化碳 (HO CO)体系对缺氧性肺动脉高压幼年大鼠肺动脉超微结构的影响。 方法 将 2 6只Wistar大鼠随机分为 4组 :对照组、低氧组、低氧 +锌原卟啉 (ZnPP)组和低氧 +一氧化碳(CO)组。以右心导管法测定肺动脉压力 ,并对大鼠肺动脉进行超微结构观察。 结果 低氧组大鼠肺动脉超微结构发生明显改变 :内皮细胞呈柱状 ,部分核突入管腔 ,排列呈栅状 ,内皮细胞肿胀 ,胞浆内可见大量空泡 ,线粒体肿胀 ,内质网扩张 ;平滑肌细胞胞体肥大 ,胞浆中肌丝和致密斑减少 ,线粒体、粗面内质网和游离核糖体等细胞器增多。约半数平滑肌细胞处于收缩表型 ,余呈过渡表型或合成表型 ,有肺血管结构重构形成。低氧 +ZnPP组肺血管结构重建程度较低氧组加重 :内皮细胞呈柱状 ,呈栅状排列 ,部分脱落致管腔 ,胞浆内可见大量空泡 ,线粒体肿胀 ,内质网扩张 ;平滑肌细胞形态不规则 ,胞体肥大 ,胞浆中肌丝和致密斑明显减少 ,粗面内质网和游离核糖体等细胞器明显增多。低氧组 +CO组肺血管结构重建程度较低氧组减轻 :内皮细胞胞体扁平 ,内衬血管内壁 ,其肿胀程度较低氧组减轻 ,空泡明显减少 ;平滑肌细胞改变较低氧组为轻 ,约半数平滑肌细胞处于收缩表型 ,余呈合成表型或过渡表型。 结论 HO CO系统对缺氧性肺?
Objective To explore the effect of hemeoxygenease/carbon monoxide system on hypoxic pulumonary vascular structural remodeling. Methods Twenty six Wister rats were randomly divided into four groups:control groups( n =6),hypoxia group( n =7),hypxia+ZnPP group( n =6) and hypoxia+CO group( n =7).Pulmonary artery mean pressure(mPAP) of each rat was elaluated by using right cardiac catheterization,the ultrastructaral changes in intra acinar pulmonary muscularized arteries were observed. Results mPAP was significantly increased in hypoxic rats(22±2 31?mmHg)as compared with that of normal controls(16 57±2 51mmHg)( P <0 01),however,mPAP was significantly increased in hypoxic rats treated with ZnPP(26 33±2 34?mmHg)as compared with that of hypoxic rats(22±2 31?mmHg)( P <0 01),mPAP was siginificantly decreased in hypoxic rats treated with CO(19 17±0 98?mmHg) as compared with that of hypoxic rats(22±2 31?mmHg)( P <0 01).Ultrastructure of pulmonary arteries changed obviously in hypoxic rats with the development of hypoxic pulmonary vasuclar structural remodeling,ZnPP increased pulmonary vascular strutral remodeling of hypoxic rats,CO ameliorated pulmonary vascular structural remodeling of hypoxic rats.Conclusion\ HO/CO plays an important role in the regulation of development of hypoxic pulmonary hypertension and hypxoic pulmonary vascular remodeling.\;[
出处
《解剖学报》
CAS
CSCD
北大核心
2003年第2期177-181,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目 ( 3 9870 844
3 0 0 70 796)
