人胎儿结肠及直肠内胰岛淀粉样多肽及5-羟色胺免疫反应细胞的研究

STUDY ON IAPP,5-HT IMMUNOREACTIVE CELLS IN THE COLON AND RECTUM OF HUMAN FETUS

目的 探讨胰岛淀粉样多肽 (IAPP)在人胎结肠、直肠中的个体发生及其与其他生物活性物质的关系。 方法 用免疫组织化学SABC法 ,对 31例 9～ 2 7周人胎结肠及直肠中IAPP免疫反应 (IR)细胞和 5 羟色胺(5 HT) IR细胞进行定位研究。 结果 人胎 9周结肠内已可见较多的 5 HT IR细胞 ,而IAPP IR细胞于 18周出现 ,直肠内 5 HT IR细胞和IAPP IR细胞均于 11周出现。随胎龄增长 ,5 HT IR细胞由少至多 ,2 0周达到高峰 ,2 1周后逐渐减少 ;而IAPP IR细胞在整个胎期始终分散存在 ,数量较少。经邻片比较观察发现 ,IAPP IR细胞与部分 5 HT IR细胞定位一致。免疫组织化学双染法显示有的细胞内IAPP与 5 HT共存。 结论 胎儿期结肠及直肠的内分泌细胞已开始合成IAPP ,并在部分细胞内IAPP和 5

Objective To investigate the ontogeny of the islet amyloid polypeptide(IAPP)and explore the relationship IAPP and other classical hormones in human fetal colon and rectum. Methods The localization of IAPP immunoreactive(IR) cells and 5 hydroxytryptamine(5 HT) IR cells in 31 human fetal colon and rectum from 9\|27 weeks of gestation was investigated with immunohistochemical SABC method. Results At 9 weeks of fetal age,a lot of 5 HT IR cells were found in the colon,but IAPP IR cells appeared at 18 weeks.In the rectum,both 5 HT and IAPP IR cells were present at 11 weeks,the number of 5 HT IR cells ascended gradually with fetus aged,reached a peak at 20 weeks and dropped gradually after 21 weeks,while in the whole fetus period,IAPP IR cells appeared fewer and scattered.The co localization of IAPP and 5 HT in some cells of colon and rectum was proved as compared with adjacent sections.Immunohistochemical double staining procedures on the same tissue section also showed that IAPP co existed with 5 HT in some cells.Conclusion\ During the human fetus,IAPP were synthesized and co existed with 5 HT in endocrine cells of the colon and rectum.The possible function on the co existence of IAPP and 5 HT in the colon and rectum of fetal was discussed. [