生殖器疱疹病毒感染检测方法的临床应用

Clinical application of laboratory methods for genital herpes virus infection

目的 : 评价检测生殖器疱疹病毒实验方法的临床应用价值。方法 : 同时采用细胞培养法、定量PCR法、间接免疫荧光法 (IIF)对 5 3例生殖器疱疹 (GH)感染患者进行了HSV检测。结果 : 细胞培养、定量PCR检测HSV阳性率明显高于IIF法 (P <0 .0 1) ,细胞培养与定量PCR比较无显著性差异 (P >0 .0 5 )。三种方法检测GH患者皮疹水疱内的阳性率无明显差异 (P >0 .0 5 )。IIF法检测糜烂结痂的阳性率仅为 2 8.6 %。结论 : 三种方法均适用GH水疱期患者标本的检查。而定量PCR。

Objective: To evaluate the value of clinical application of laboratory methods for genital herpes simples virus (HSV). Methods: HSV was detected with cell culture, quantity PCR and indirect immunofluorescene (IIF) in 53 cases with clinically diagnosed genital herpes (GH). Results: HSV positive rates by cell culture and quantity PCR were higher than that with IIF(P<0.01). There was no significant difference in the positive rates among the three methods, when the specimens were taken from clinical blisters (P>0.05). The positive rate of the specimen tested from erosive lesions with IIF was only 28.6%. Conclusion : All three methods are suitable for the specimens taken from clinical GH blisters in detection of HSV. When the specimens taken from erosive lesions, quantity PCR and cell culture are more sensitive than IIF method.