树突状细胞对肿瘤浸润性淋巴细胞抗结肠癌活性影响的研究

Study of Anti-carcinoma of Colon Activity of Tumor Infiltrating Lymphocytes Affected by Dendritic Cells

目的:探讨树突状细胞(DC)激活的肿瘤浸润性淋巴细胞(TIL)体外对结肠癌细胞的杀伤活性。方法:从结肠癌患者外周血获取DC,应用粒/巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-4(IL-4)和肿瘤抗原激活DC,然后用DC激活TIL,观察TIL在体外对自体结肠癌细胞和VoLo细胞的杀伤活性。结果:DC激活的TIL对自体结肠癌细胞具有很强的杀伤活性,杀伤率为87.62%±3.01%,明显高于未经DC激活的TIL、DC激活的T淋巴细胞和未经DC激活的T淋巴细胞对自体结肠癌细胞的杀伤率分别为53.72%±1.50%、52.23%±1.46%和3.55%±0.25%,而它们对VoLo细胞的杀伤活性则相对较低。结论:结肠癌患者外周血DC能诱导TIL产生高效而特异的抗结肠癌免疫活性。

Objective:To study the killing activity of tumor infiltrating lymphocytes(TIL)stim ulated by dendritic cells(DC)on carcinoma of colon cells in vitro.Methods:DCs were iso-lated from peripheral blood of patients with carcinoma of colon.DCs were stimulated by granulo-cyte /macrophage colony stimulating factor(GM-CSF),interleukin-4(IL-4)and tumor antigen.Then TIL were stimulated by DCs and their killing activity on autogenous carcinoma of colon cells and VoLo cells in vitro were observed.Results:TIL stimulated by DCs had very high killing activity on autogenous carcinoma of colon cells and the killing rate was87.62%±3.01%.The killing rate was higher obviously than that of TIL not stimulated by DC and T lymphocytes stimulated by DC or not on autogenous carcinoma of colon cells,respectively(killing rate:53.72%±1.50%,52.23%±1.46%and3.55%±0.25%,respectively).But their killing activity on VoLo cells was lower.Conclusion:The results indicate that DC from patients with carcinoma of colon can in duce efficient and specific anti-carcinoma of colon immune response.