人乳腺癌组织中环氧化酶-2的表达

Expression of the cyclooxygenase-2 gene in human breast carcinoma

目的 探讨环氧化酶 2 (COX 2 )在乳腺癌组织、正常腺体及乳腺良性肿瘤中的表达 ,COX 2与雌激素受体 (ER)表达的关系及COX 2在乳腺癌发生发展中的意义。 方法 以 β actin基因为参照 ,应用逆转录聚合酶链反应 (RT PCR)技术检测 30例乳腺癌、相应癌旁正常腺体及 15例乳腺良性肿瘤中COX 2mRNA的表达。应用条带密度分析软件定量分析RT PCR产物电泳带密度。应用免疫组化方法测定 30例乳腺癌中ER表达情况。 结果 COX 2mRNA在 30例癌组织中有 2 8例表达水平明显增高 ,全距 0 0 5～ 0 91,中位数 0 5 3;正常腺体中无表达或表达很弱 ,全距为 0～0 0 9,中位数为 0 ;在部分良性肿瘤中表达明显增高 ,全距 0～ 0 6 8,中位数为 0 0 7。癌组织与正常腺体或良性肿瘤间COX 2的表达差异有显著性意义 (秩和检验 ,P <0 0 5 )。ER阳性组与ER阴性组的COX 2表达差异有非常显著性意义 (秩和检验 ,P <0 0 1)。 结论 乳腺癌组织中COX 2mRNA表达水平高于正常腺体或乳腺良性肿瘤 ,激素依赖型乳腺癌中COX 2表达高于非激素依赖型乳腺癌 ,其表达在乳腺癌的发生、发展中具有重要意义。

ObjectiveTo study the expression of the cyclooxygenase-2(COX-2) gene in breast cancer in contrast to that of normal breast tissues or benign breast tumors and its significance in the carcinogenesis and development of breast cancer.MethodsWith reference to the expression of the β-actin gene,the expression of COX-2 mRNA was examined in cancerous tissues and adjacent normal breast tissue from 30 patients and benign breast tumors from 15 patients by reverse transcription-polymerase chain reaction(RT-PCR). Quantitation of relative band densities was performed using densitometry-scanning software. Estrogen receptors of 30 breast cancers were investigated by immunohistochemistry.Results Enhanced expression of COX-2 was observed in ninety percent of cancers tissue with a range of 0.05-0.91(median 0.53). Rare cases showed significant COX-2 expression in normal breast tissues with a range of 0-0.09(median 0). In part of benign breast tumors, COX-2 expressions were obviously elevated with a range of 0-0.68(median 0.07). The difference of expression of COX-2 mRNA among breast cancers, nomal breast tissues, mastopathy or fibroadenomas was significant(rank-sum test, P <0.05) and the difference of that between estrogen receptor negtive and positive was also observed(rank-sum test, P <0.01).ConclusionThe level of expression of COX-2 mRNA is obviously higher in breast cancer tissue than in normal breast tissue, mastopathy or fibroadenomas. The expression of COX-2 in hormone-dependent breast cancer is higher than that in hormone-independent breast cancer. The overexpression of COX-2 may play a crucial role in the carcinogenesis and development of cancer in patients with breast carcinoma.