犬急性肝衰竭一种新模型的建立

Establishment of a new canine model of acute hepatic failure

目的 为进行生物人工肝治疗等研究制备一种新的犬急性肝衰竭模型。方法 采用一期门腔端 -侧分流、胆总管切断结扎术制作模型。观察其生存情况、肝功能、血氨、Fischer比 (支链氨基酸 /芳香氨基酸 ,BCAA /AAA ) ,并观察模型肝脏病理变化。结果 模型犬于术后血氨、谷丙转氨酶(ALT )、碱性磷酸酶 (ALP)、总胆红素 (TB)逐渐升高 ,凝血酶原时间 (PT)延长 ,Fischer比下降 ,于 2周时最为明显 ;14d后随着时间的延长 ,动物逐渐死亡。肝脏病理检查显示典型的淤胆、汇管区炎症、肝细胞变性、坏死表现。结论 一期门腔端 -侧分流、胆总管结扎切断术制作犬急性肝衰竭模型成功 ,该模型可望用于生物人工肝的研究。

Objective To establish a new model of acute hepatic failure in canines for bioartificial liver treatment research . Methods Acute hepatic failure was induced by end to side portocaval shunt combined with common bile duct ligation and transection. The survival rate, liver function, ammonia, Fischer's ratio (BCAA/AAA) and pathological change of injury liver were investigated. Results The ammonia, ALT, AKP, total bilirubin and PT were elevated dramatically and Fischer's ratio declined significantly 14 days after the operation. The survival rate of the models decreased with time 14 days after the operation. Pathological examination of liver tissues revealed evidence of cholestasis, inflammation with accumulation of neutrophils, degeneration and necrosis of hepatocytes.Conclusions End to side portocaval shunt combined with common bile duct ligation and transection can produce a satisfactory acute hepatic failure model in canines, which could be used in the study of bioartificial liver.