老年和成年APP转基因小鼠脑蛋白质组2-DE图谱比较

Comparison of the two-dimensional electrophoresis patterns between APP transgenic mice and control mice brains in proteomatic analysis

目的:比较老年和成年APP转基因小鼠脑蛋白质组双向电泳(2-DE)图谱差异,从蛋白质水平初步探索老年痴呆发病机制。方法:以固相pH梯度等电聚焦(IPG)为第一向,SDS-PAGE垂直电泳为第二向进行2-DE。图象分析软件Imagemaster 2D Elite分析电泳图谱。结果:老年和成年转基因小鼠脑组织2-DE图谱分别检出964和947个蛋白点。对两张电泳图进行匹配后,发现有17个蛋白点仅在老年转基因小鼠脑蛋白2-DE图谱检测到表达,而有5个蛋白点只在成年转基因小鼠检测到。部分蛋白在2组小鼠脑组织中含量发生了明显变化。结论:初步建立了AD动物模型差异表达蛋白质组学的技术方法;差异点的发现为研究AD机理及治疗AD提供了有益的线索。

Objective: To investigate molecular mechanisms of Alzheimer's disease, the two-dimensional electrophoresis (2-DE) was employed to compare the global protein patterns between adult and aged APP transgenic mouse brains. Methods: Two hundred micrograms of mouse brain proteins was run immobilized pH gradient (IPG) isoelectric focusing electrophoresis as the first dimension, and then run vertical SDS-PAGE as the second dimension. The map is visualized by Silver staining and analy-sised with Image Master 2D Elite software. Results: Averagely, 964 and 947 protein spots were obtained in aged and adult APP transgenic mouse brain map, of which 42 spots increased or decreased in quantity. Another 17 and 5 spots were exclusively showed in aged and adult APP transgenic mice brain map, respectively. Conclusions: The differentially displayed proteins between aged and adult APP transgenic mice brains are quite useful for diagnosing and treatment Alzheimer disease.