摘要
选用常用的两种报告基因、两种抗性基因、两种启动子序列和两种终止子序列为探针,将其PCR扩增产物用MicroGridⅡ型全自动点样仪按矩阵排列点样于包埋有氨基的载玻片上,制备成转基因作物检测型基因芯片。利用该芯片对4种转基因水稻、木瓜、大豆、玉米进行检测,结果表明,该芯片能对转基因作物做出快速、准确的检测。
Some selected available sequences of reporter genes,resistant genes,promoters and terminators are amplified by PCR for the probes of transgenic crop detection gene chip.These probes are arrayed at definite density and printed on the surface of amino-slides by bioRobot MicroGrid Ⅱ.Results showed that gene chip worked quickly and correctly,when transgenic rice,pawpaw,maize and soybean were applied.
出处
《遗传》
CAS
CSCD
北大核心
2003年第3期307-310,共4页
Hereditas（Beijing)
