肺肿瘤和正常组织自体荧光与激发光波长的关系

Autofluorescence of Tumor and Lung Tissue Versus Excitation Wavelength

人体肿瘤,如支气管粘膜肿瘤的自体荧光与正常组织的自体荧光存在差异,据此进行肿瘤的检测是可能的。但是,人们还不清楚这种差异性是否足够大,以及什么是最佳的激发波长和发射波长。作者测量了人肺肿瘤试样的自体荧光激发光谱,分别在520nm和690nm2种发射波长处测得激发谱与300nm到600nm激发波长的函数关系。用405nm和一些更长的波长激发,也测量了人肺组织和肿瘤试样在500nm到750nm波长范围的自体荧光发射谱。在略小于约600nm的一段光谱区,肿瘤发射的荧光小于正常组织的荧光。结果表明,单独用二者自体荧光的差异有可能提供一种探测肿瘤的新方法。

Detection of human tumors, e g. of the bronchial mucosa, by differences in autofluoresceuce of tumor and normal tissue, is a possibility. It is not known if the differences are sufficiently large, or what might be the optimum excitation and emission wavelengths The autofluorescence excitation spectra of human lung tumor specimens has been measured as a function of excitation wavelength from 300 nm to 600 nm, at emissiou wavelengths of 520 nm and 690 nm. The emission spectra of human lung tissue and tumorspecimens were measured from 500 nm to 750 nm, for excitation at 405 nm and longer wa, vclengths The emission at wavelengths below some 600 nm er so is less in the tumor than in normal lung tissue This difference may afford a method of imaging or detectilng tumors by autofluorescencc alone