肾病综合征患儿血清中细胞因子测定的临床意义

Detecting significance of serum cytokines in the children with nephrotic syndrome

目的探讨肾病综合征(NS)患儿血清中白细胞介素-1(IL-1),白细胞介素-6(IL-6),白细胞介素-8(IL-8)和肿瘤坏死因子α(TNFα)的测定的临床意义。方法将24例NS患儿分为激素治疗前和治疗后两组,应用间接酶联免疫吸附试验(ELISA)方法检测血清中IL-1,IL-6,IL-8和TNFα的水平。结果NS患儿激素治疗前与治疗后比较血清中IL-1(195±25vs120±25),IL-6(355±62vs200±23),IL-8(286±55vs178±32)和TNFα(265±36vs110±40)的水平测定,两组差异有显著性意义(P<0.01)。结论血清中多种细胞因子参与肾病综合征NS的发病,动态监测IL-1,IL-6,IL-8和TNFα含量可以作为判断疾病程度的免疫学指标。

Objective To explore the clinical significance of serum IL-1,IL-6,IL-8and TNF α in the children with nephrotic syndrome(NS).Methods Serum IL-1,IL-6,IL-8and TNF α were detected by ELISA in24children with NS before and after treatment with glucocorticoid.Results Significant differences of IL-1(195±25vs120±25),IL-6(355±62vs200±23),IL-8(286±55vs178±32)and TNF α (265±36vs110±40)were showed between two groups before and after treatment(P<0.01).Conclusions Serum IL-1,IL-6,IL-8and TNF α take some actions to nephrotic syndrome.The concentrations of serum cytokines may be indexes for diagnosing activation of NS.