白念珠菌CDR1基因表达与氟康唑耐药的关系

Relevance of CDR1 gene expression of Candida albicans and fluconazole resistance

目的 了解在白念珠菌氟康唑耐药或敏感菌株中CDR1基因的表达情况。方法 抽提氟康唑耐药 /敏感菌株的RNA ,采用Northernblot技术观察CDR1基因的表达。结果 白念珠菌耐药菌株CDR1基因的表达量明显高于对应的敏感菌株。结论 白念珠菌CDR1基因的高表达与氟康唑耐药有关。

Objective To explore the relevance of fluconazole resistance and expression of the CDR1 gene encoding the membrane transport proteins of the ABC transporter. Methods The RNA of fluconazole-susceptible and fluconazole-resistant Candida albicans isolates were extracted, and the expression of the CDR1 gene was observed by Northern blot method. Results Higher expression of CDR1 gene was observed in the fluconazole-resistant isolates than that on the fluconazole-susceptible isolates. Conclusions It is likely to suggest that over-expression of CDR1 gene of Candida albicans is relevant to fluconazole resistance.