柿叶黄酮对晚期氧化蛋白产物诱导的血管外膜成纤维细胞增殖的影响

Effects of Flavone from Leaves of Diospyros kaki on Adventitial Fibroblasts Proliferation by Advanced Oxidation Protein Products in Vitro

目的 :观察柿叶黄酮对晚期氧化蛋白产物 (AOPP)诱导的血管外膜成纤维细胞增殖的影响。方法 :体外培养NIH 3T3细胞 ,应用柿叶黄酮与晚期氧化蛋白产物共同进行作用并设立对照进行比较 ,采用非放射性的MTS/PES法确定成纤维细胞的增殖状态。结果 :各组的细胞增殖率分别为对照组 (1 789± 0 2 99) ;AOPP 10 0 μg/ml组(2 0 6 4± 0 14 1) ;AOPP 5 0 μg/ml组 (2 14 9± 0 2 18) ;AOPP 10 μg/ml组 (2 10 8± 0 16 5 ) ,AOPP 1μg/ml组 (2 12 4±0 131) ;AOPP 10 0ng/ml组 (2 0 87± 0 12 5 ) ;柿叶黄酮 5 0 μg/ml组 (1 714± 0 179) ;AOPP 5 0 μg/ml联用柿叶黄酮 5 0μg/ml组 (1 80 7± 0 10 5 )。统计分析显示低至 10 0ng/ml的AOPP仍能明显刺激成纤维细胞的增殖 (P <0 0 5 )。柿叶黄酮单独作用对成纤维细胞增殖率无影响 (P >0 0 5 ) ;柿叶黄酮对AOPP诱导的成纤维细胞增殖有明显的抑制作用(P <0 0 5 )。结论 :柿叶黄酮能明显抑制由晚期氧化蛋白产物刺激的血管外膜成纤维细胞增殖。

Objective: To observe the effects of flavone from leaves of Diospyros kaki on adventitial fibroblasts proliferation by advanced oxidation protein products (AOPP) in vitro.Methods: NIH-3T3 cells were cultured in vitro and treated with AOPP and flavone from leaves of Diospyros kaki,respectively,and observed in comparison with the control group.The ratio of cell proliferation was determined by non-radioactive MTS/PES assay.Results: The ratio of cell proliferation was 1.789±0.299 in the control group,and 2.064±0.141,2.149±0.218,2.108±0.165,2.124±0.131 and 2.087±0.125 in AOPP groups corresponding to AOPP concentrations of 100,50,10,1 and 0.1 μg/ml, respectively.It showed that AOPP significantly induced the fibroblasts proliferation when the concentration was above 100 ng/ml (P<0.05).The ratio of cell proliferation was 1.714±0.179 in flavone from leaves of Diospyros kaki group corresponding to concentration of 50 μg/ml.It also showed that flavone from leaves of Diospyros kaki alone had no effect on fibroblasts proliferation(P>0.05).With AOPP stimulation,flavone from leaves of Diospyros kaki significantly inhibited fibroblasts proliferation (P<0.05).Conclusions: Flavone from leaves of Diospyros kaki can significantly inhibit the adventitial fibroblasts proliferation stimulated by AOPP in vitro.