Dot—ELISA检测抗丝虫抗体的初步实验研究

PRELIMINARY STUDIES ON DETECTION OF ANTI—FILARIAL ANTIBODIES BY DOT—ELISA

本文报告以马来丝虫成虫可溶性抗原作Dot-ELISA检测丝虫病人抗丝虫抗体的初步结果。62例马来丝虫微丝蚴血症者的阳性符合率为96.8％,49例班氏丝虫微丝蚴血症者的阳性符合率为85.7％,50例和39例非流行区正常人的假阳性率分别为4.0％和5.1％,与钩虫和蛔虫混合感染者的血清有一定的交叉反应。实验结果显示,Dot—ELISA检测微丝蚴血症者抗丝虫抗体具有敏感性高,且方法简便,可望用于丝虫病监测。

Dot—ELISA was used in the detection of anti—filarial antibodies of filariasis patients with the soluble antigen of Brugia malayi adult worm. The positive rates of the sera from 62 B. malayi microfilaremia and 49 Wuchereria bancrofti microfilaremia were 96.80％ and 85.7％ respectively; while in 50 and 39 healthy individuals the false positive rates were 4％ and 5.1％ respectively and 17.90％ cross reaction rate with hookworm and Ascaris infection were observed. The results indicated that this method was highly sensitive, simple and convenient, and might be suitable for serological surveillance of filariasis.