摘要
用盲肠内接种阿米巴滋养体的方法,复制出大鼠肠阿米巴病动物模型,经组织学及扫描电镜观察发现:感染大鼠的盲肠病变与人类急慢性肠阿米巴病类似,早期为盲肠的弥漫性、非特异性炎症及溃疡形成,晚期为修复愈合与新鲜溃疡同时存在的组织学改变,病变出现高峰在接种后3～7d 间;大鼠感染率为93.6%,盲肠溃疡发生率为43.6%,可作为研究肠阿米巴病的动物模型。应用自行制备的兔抗阿米巴血清对感染的盲肠组织进行免疫组织化学(PAP)染色的结果,证实此法能清楚、准确地显示组织中的阿米巴滋养体,可用于肠阿米巴病的病理诊断及回顾性研究。
An animal model of intestinal amoebiasis was established by inoculating Entamoeba histolyticatrophozoites into the caecum of Wistar rats.By light and scanning electronic microscopy,it is found that theearly pathological changes in rat caecum were diffuse,nonspecific inflammation and ulceration.In the latestage both long standing lesions with reparative changes and fresh ulcer were simutaneously present.The in-fection rate was 93.6% and about 43.6% rats showed caecal ulceration that reached its peak 3—7days afterthe inoculation.Anti—amoebae serum was prepared in rabbits and used to demonstrated amoeba in tissues byimmunohistochemistry(PAP)technique.By this method,the amoeba trophozoites could be much more clear-ly and certainly identified.The PAP method could be of help in diagnosis and retrostrated studies of amoebi-asis.
出处
《中国寄生虫病防治杂志》
CSCD
1992年第4期260-263,共4页
Chinese Journal of Parasitic Disease Control
关键词
阿米巴病
病理学
扫描电镜
Amoebiasis
pathology
scanning electron microscopy
immuno-histochemistry
