聚合酶链反应检测耐甲氧西林葡萄球菌的mecA基因

PCR for Detecting mecA Gene of MRS

目的 应用聚合酶链反应技术 ( PCR)快速检测耐甲氧西林葡萄球菌 ( MRS)的 mec A基因 ,与相关抗生素药敏表型试验对照分析 ,为临床合理使用抗生素提供实验室依据。方法 根据文献我室自行设计的一对引物[1],建立了 MRS的 mec A基因的 PCR检验法 ,检测我院临床各科分离到的 52株葡萄球菌标本 ,同时进行药敏试验和 m ec A基因的 PCR法检测。结果 PCR检测 52株葡萄球菌 mec A基因 38株阳性 ,耐苯唑西林 ( OX)的葡萄球菌 32株中 ,mec A基因阴性 1株。苯唑西林敏感而 m ec A阳性 7株。两种方法经 χ2检验结果差异无显著性 ( P>0 .50 )。结论 mec

Objective To detect mecA gene of MRS(methicillin resistant staphylococcus) with PCR and to compare the results with conventional microbiologic method so as to provide the scientific basis for reasonable application of antibiotics. Methods PCR with a pair of primer based on relevent literature was carried out among 52 strains of MRS isolated from our hospital and the results were compared with drug sensitivity test. Results The results were as follows: among 52 strains detected, 38 strains showed positive in mecA gene and in which 32 strains resistant to OX; among 32 strains resistant to OX, 1 strain mecA gene negative; 7 strains sensitive to OX but positive in mecA gene. There were no significant differences between PCR and drug sensitivity test ( P >0.05) Conclusion PCR for detecting mecA gene of MRS would have similar clinical significance with staphylococci resistant OX.